高精度实时主动轴向防漂移系统研究

霍英东; 曹博; 于斌; 陈丹妮; 牛憨笨

doi:10.7498/aps.64.028701

摘要

基于单分子定位的荧光纳米分辨显微成像中, 系统漂移会使得单分子定位出现额外偏差, 从而使重构图像的分辨率降低, 造成图像模糊. 因此, 对系统漂移量的控制至关重要. 近年来, 防漂移的方法层出不穷. 本文针对其中一种利用光学测量原理和引入负反馈的防漂移方法做了系统的研究, 分析了其原理和实现过程, 对整个系统进行了误差分析, 通过实验标定了整个防漂移系统的精度. 该系统可以主动实时地校正漂移量, 实现了显微镜轴向9.93 nm的防漂移精度. 与现有商用的显微镜自带的防漂移装置相比, 防漂移精度提高了一个量级.

关键词:

Abstract

In a fluorescent nano-resolution microscope based on single molecular localization, drift of focal plane will bring an additional deviation to the accuracy of single molecular localization. Consequently, this will reduce the final resolution of the reconstructed image and cause image degradation. Therefore, it is vital to control the system drift to a minimum level as much as possible. In recent years, the anti-drift ways emerged in endlessly. In this paper we made a systematic study aiming at the method in which optical measurement and negative feedback control are used. The basic principle and its implementation of the system are analyzed, and possible error is also evaluated. Finally, the precision of the system is tested experimentally. With this device, axial drift can be detected and corrected automatically in time, and the axial anti-drift accuracy as high as 9.93 nm can be achieved, which is one order higher than that of the existing commercial microscopies.

Keywords:

作者及机构信息

1.
中国科学院西安光学精密机械研究所, 超快诊断技术中国科学院重点实验室, 西安 710119;

2.
深圳大学光电工程学院, 光电子器件与系统(教育部、广东省) 重点实验室, 深圳 518060;

3.
清华大学精密测试技术及仪器国家重点实验室, 北京 100084

基金项目: 国家重点基础研究发展计划 (批准号: 2012CB825802, 2015CB352005)、国家自然科学基金 (批准号: 61335001, 61235012, 61178080, 11004136, 60878053)、国家重大科学仪器设备开发专项 (批准号: 2012YQ15009203)和深圳市科技计划 (批准号: ZYC201105170233A, JCYJ20120613173049560)资助的课题.

Authors and contacts

1.
CAS Key Laboratory of Ultrafast Diagnostics, Xi'an Institute of Optics and Precision Mechanics of CAS, Xi'an 710119, China;

2.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China;

3.
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China

Funds: Project supported by the National Basic Research Program of China (Grant Nos. 2012CB825802, 2015CB352005), the National Natural Science Foundation of China (Grant Nos. 61335001, 61235012, 61178080, 11004136, 60878053), the Special Funds of the Major Scientific Instruments Equipment Development of China (Grant No. 2012YQ15009203), and the Science and Technology Planning of Shenzhen, China (Grant Nos. ZYC201105170233A, JCYJ20120613173049560).

参考文献

[1]	Huang B, Wang W, Bates M, Zhuang X 2008 Science 319 810
[2]	Pavani S R P, Thompson M A, Biteen J S, Lord S J, Liu N, Twieg R J, Piestun R, Moerner W 2009 PNAS 106 2995
[3]	Juette M F, Gould T J, Lessard M D, Mlodzianoski M J, Nagpure B S, Bennett B T, Hess S T, Bewersdorf J 2008 Nat. Methods 5 527
[4]	Rust M J, Bates M, Zhuang X 2006 Nat. Methods 3 793
[5]	Chen D N, Liu L, Yu B, Niu H B 2010 Acta Phys. Sin. 59 6948 (in Chinese) [陈丹妮, 刘磊, 于斌, 牛憨笨 2010 物理学报 59 6948]
[6]	Shtengel G, Galbraith J A, Galbraith C G, Lippincott-Schwartz J, Gillette J M, Manley S, Sougrat R, Waterman C M, Kanchanawong P, Davidson M W 2009 PNAS 106 3125
[7]	Ram S, Prabhat P, Ward E S, Ober R J 2009 Opt. Express 17 6881
[8]	Li H, Yu B, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 124201 (in Chinese) [李恒, 于斌, 陈丹妮, 牛憨笨 2013 物理学报 62 124201]
[9]	Yu B, Li H, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 154206 (in Chinese) [于斌, 李恒, 陈丹妮, 牛憨笨 2013 物理学报 62 154206]
[10]	Shroff H, Galbraith C G, Galbraith J A, Betzig E 2008 Nat. Methods 5 417
[11]	Bates M, Huang B, Dempsey G T, Zhuang X 2007 Science 317 1749
[12]	Gould T J, Verkhusha V V, Hess S T 2009 Nat. Protoc. 4 291
[13]	Peters J 2008 Nat. Methods, Application Notes
[14]	Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S, Davidson M W, Lippincott-Schwartz J, Hess H F 2006 Science 313 1642
[15]	Carter A R, King G M, Ulrich T A, Halsey W, Alchenberger D, Perkins T T 2007 Appl. Opt. 46 421
[16]	Lee S H, Baday M, Tjioe M, Simonson P D, Zhang R, Cai E, Selvin P R 2012 Opt. Express 20 12177
[17]	Mcgorthy R, Kamiyama D, Huang B 2013 Opt. Nanosc. 2 3
[18]	Wade A, Fitzke F 1998 Opt. Express 3 190
[19]	Mlodzianoski M J, Schreiner J M, Callahan S P, Smolková K, Dlasková A, Šantorová J, Ježek P, Bewersdorf J 2011 Opt. Express 19 15009

施引文献

[1]	Huang B, Wang W, Bates M, Zhuang X 2008 Science 319 810
[2]	Pavani S R P, Thompson M A, Biteen J S, Lord S J, Liu N, Twieg R J, Piestun R, Moerner W 2009 PNAS 106 2995
[3]	Juette M F, Gould T J, Lessard M D, Mlodzianoski M J, Nagpure B S, Bennett B T, Hess S T, Bewersdorf J 2008 Nat. Methods 5 527
[4]	Rust M J, Bates M, Zhuang X 2006 Nat. Methods 3 793
[5]	Chen D N, Liu L, Yu B, Niu H B 2010 Acta Phys. Sin. 59 6948 (in Chinese) [陈丹妮, 刘磊, 于斌, 牛憨笨 2010 物理学报 59 6948]
[6]	Shtengel G, Galbraith J A, Galbraith C G, Lippincott-Schwartz J, Gillette J M, Manley S, Sougrat R, Waterman C M, Kanchanawong P, Davidson M W 2009 PNAS 106 3125
[7]	Ram S, Prabhat P, Ward E S, Ober R J 2009 Opt. Express 17 6881
[8]	Li H, Yu B, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 124201 (in Chinese) [李恒, 于斌, 陈丹妮, 牛憨笨 2013 物理学报 62 124201]
[9]	Yu B, Li H, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 154206 (in Chinese) [于斌, 李恒, 陈丹妮, 牛憨笨 2013 物理学报 62 154206]
[10]	Shroff H, Galbraith C G, Galbraith J A, Betzig E 2008 Nat. Methods 5 417
[11]	Bates M, Huang B, Dempsey G T, Zhuang X 2007 Science 317 1749
[12]	Gould T J, Verkhusha V V, Hess S T 2009 Nat. Protoc. 4 291
[13]	Peters J 2008 Nat. Methods, Application Notes
[14]	Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S, Davidson M W, Lippincott-Schwartz J, Hess H F 2006 Science 313 1642
[15]	Carter A R, King G M, Ulrich T A, Halsey W, Alchenberger D, Perkins T T 2007 Appl. Opt. 46 421
[16]	Lee S H, Baday M, Tjioe M, Simonson P D, Zhang R, Cai E, Selvin P R 2012 Opt. Express 20 12177
[17]	Mcgorthy R, Kamiyama D, Huang B 2013 Opt. Nanosc. 2 3
[18]	Wade A, Fitzke F 1998 Opt. Express 3 190
[19]	Mlodzianoski M J, Schreiner J M, Callahan S P, Smolková K, Dlasková A, Šantorová J, Ježek P, Bewersdorf J 2011 Opt. Express 19 15009

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