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| A nnovel wide field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination |
| Wen Qiao, Wang Kai-Ge, Shao Yong-Hong, Qu Jun-Le, Niu Han-Ben |
| Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China |
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Abstract We propose a novel wide-field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination in this article. A new type of polarization filtering image enhancement technology, based on the differences in polarization characteristic between excitation light and fluorescence, is used to filter out the excitation light and enhance image quality. The dynamic speckle illumination is employed to achieve the wide field fluorescence sectioning which has many advantages, such as simple configuration, low cost, rapid response and easy operation. The experimental results indicate that our filtering method can be utilized to improve the image quality significantly, and the dynamic using speckle illumination can be employed to achieve optical sectioning with a wide field and a high vertical resolution capability. Our study is not only to enrich the technological method to extract the weak fluorescence signal from the strong excitation light, but also to provide a significant reference for developing a light wavelength tunable multi-spectral fluorescence microscope with rapid response.
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Received: 21 May 2012
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| Fund:Project supported by the National Natural Science Foundation of China (Grant Nos. 61108026, 61078067), Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20114408120001), Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (Grant No. LYM11107) and Science Foundation of Shenzhen University, China (Grant No. 201033). |
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Corresponding Authors:
屈军乐
E-mail: jlqu@szu.edu.cn
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2013, Vol. 62 
