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Plant fluorescence is a susceptible signal in plant fluorescence remote sensing detection. In order to solve this problem, a technique for plant chlorophyll fluorescence lifetime imaging is presented to evaluate living status for plant growth and environmental monitoring. A concave lens is used to expand laser beam at a wavelength of 355 nm, and the living plant is exposed in this laser light source to excite chlorophyll fluorescence. And the chlorophyll fluorescence signals are detected by an intensification charge coupled device. Time resolved measurement method is used in this article, so that every time the same fluorescence signals can be excited by the same laser pulse. Meanwhile, the delay time needed for triggering intensification charge coupled device should be changed consecutively, and the whole discrete fluorescence signal can be obtained. The discrete fluorescence signals from the particular location points of the plant are fitted. An improved method of forward iterative deconvolution is used to retrieve the corresponding fluorescence lifetime, and the high-precision fluorescence lifetime can be obtained. Furthermore, the fluorescence lifetime values at all the location points are retrieved to obtain the distribution map of chlorophyll fluorescence lifetime. This method can give the chlorophyll fluorescence image efficiently. The distribution map of fluorescence lifetime can more effectively reflect the plant chlorophyll concentration than the fluorescence intensity image does. The physical property of chlorophyll fluorescence lifetime from living plants has been studied preliminarily, indicating that the plant physiological status is related to its fluorescence lifetime to a certain extent; and the chlorophyll fluorescence lifetime and plant environment have a subtle and complex correlation. In the future, the relationship between chlorophyll fluorescence lifetime and plant environment will be expected to study with the cooperation of biophysicist.
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Keywords:
- fluorescence lifetime imaging /
- laser-induced fluorescence /
- fluorescence LiDAR /
- chlorophyll
[1] Janusauskaite D, Feiziene D 2002 Acta. Agr. Scand. B-S. P. 62 7
[2] Tol C, Verhoef W, Rosema A 2009 Agr. Forest Meteorol. 149 96
[3] Men Z W, Fang W H, Li Z W, Qu G N, Gao S Q, Lu G H, Yang J G, Sun C L 2010 Chin. Phys. B 19 8
[4] Fu C Y, Ng B K, Razul S G 2009 J. Biomed Opt. 14 064009
[5] Zhao M, Peng L L 2010 Opt. Lett. 35 2910
[6] Jordi R, Michael S, Santiago R 2012 Opt. Lett. 37 1229
[7] Hungerford G, Birch D J S 1996 Meas. Sci. Technol. 7 121
[8] Yuan S, Chin S L, Zeng H P 2015 Chin. Phys. B 24 1
[9] Roberts M S, Dancik Y, Prow T W, Thorling C A, Lin L L, Grice J E, Robertson T A, Konig K, Becker W 2011 Eur. J. Pharm Biopharm 77 469
[10] Gutierrez-Navarro O, Campos-Delgado D U, Arce-Santana E R, Maitland K C, Cheng S, Jabbour J, Malik B, Cuenca R, Jo J A 2014 Optics Express 22 12255
[11] Miao Z, Li S F, Zhang Q Y 2006 Acta Phys. Sin. 55 4321 (in Chinese) [苗壮, 李善锋, 张庆瑜 2006 物理学报 55 4321]
[12] Alex S, Mary L 2014 Opt Lett 39 5362
[13] Oliveira F F, Ito A S, Bachmann L 2010 Appl. Optics 49 2244
[14] Wei H Y, Xu T, Wang F, Peng X S, Wei X, Liu S Y 2013 Acta Optica Sinica 33 0823001 (in Chinese) [魏惠月, 徐涛, 王峰, 彭晓世, 韦欣, 刘慎业 2013 光学学报 33 0823001]
[15] Garca M, Vzquez R, Isakina S, Lpez R 2012 Electrical Communications and Computers 27 317
[16] Kennifer R D, Peter M A T 2003 Practical applications of chlorophyll fluorescence in plant biology (Holland: Kluwer Academic Publishers) pp109-132
[17] Kotzagianni M, Couris S 2013 Chemical Physics Letters 561 36
[18] Xu M F, Shi Y B, Gao W H, Chen H Y 2014 Chinese Journal of Lasers 41 0108005 (in Chinese) [徐美芳, 石云波, 高文宏, 陈海洋 2014 中国激光 41 0108005]
[19] Mukamel E A, Babcock H, Zhuang X W 2012 Biophysical Journal 102 2391
[20] Wan W B, Hua D X, Le J, Liu M X, Cao N 2013 Acta Phys. Sin. 62 190601(in Chinese) [万文博, 华灯鑫, 乐静, 刘美霞, 曹宁 2013 物理学报 62 190601]
[21] Lichtenthaler H K 2011 Applications of chlorophyll fluorescene (Holland: Kluwer Academic Publishers) pp182-196
[22] Kumar P J, Gopal R 2011 Spectroscopy-Biomedical Applications 26 129
[23] Fang W H, Li Z W, Li Z L, Qu G N, Ouyang S L, Men Z W 2012 Acta Phys. Sin. 61 153301(in Chinese) [房文汇, 里佐威, 李占龙, 曲冠男, 欧阳顺利, 门志伟 2012 物理学报 61 153301]
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[1] Janusauskaite D, Feiziene D 2002 Acta. Agr. Scand. B-S. P. 62 7
[2] Tol C, Verhoef W, Rosema A 2009 Agr. Forest Meteorol. 149 96
[3] Men Z W, Fang W H, Li Z W, Qu G N, Gao S Q, Lu G H, Yang J G, Sun C L 2010 Chin. Phys. B 19 8
[4] Fu C Y, Ng B K, Razul S G 2009 J. Biomed Opt. 14 064009
[5] Zhao M, Peng L L 2010 Opt. Lett. 35 2910
[6] Jordi R, Michael S, Santiago R 2012 Opt. Lett. 37 1229
[7] Hungerford G, Birch D J S 1996 Meas. Sci. Technol. 7 121
[8] Yuan S, Chin S L, Zeng H P 2015 Chin. Phys. B 24 1
[9] Roberts M S, Dancik Y, Prow T W, Thorling C A, Lin L L, Grice J E, Robertson T A, Konig K, Becker W 2011 Eur. J. Pharm Biopharm 77 469
[10] Gutierrez-Navarro O, Campos-Delgado D U, Arce-Santana E R, Maitland K C, Cheng S, Jabbour J, Malik B, Cuenca R, Jo J A 2014 Optics Express 22 12255
[11] Miao Z, Li S F, Zhang Q Y 2006 Acta Phys. Sin. 55 4321 (in Chinese) [苗壮, 李善锋, 张庆瑜 2006 物理学报 55 4321]
[12] Alex S, Mary L 2014 Opt Lett 39 5362
[13] Oliveira F F, Ito A S, Bachmann L 2010 Appl. Optics 49 2244
[14] Wei H Y, Xu T, Wang F, Peng X S, Wei X, Liu S Y 2013 Acta Optica Sinica 33 0823001 (in Chinese) [魏惠月, 徐涛, 王峰, 彭晓世, 韦欣, 刘慎业 2013 光学学报 33 0823001]
[15] Garca M, Vzquez R, Isakina S, Lpez R 2012 Electrical Communications and Computers 27 317
[16] Kennifer R D, Peter M A T 2003 Practical applications of chlorophyll fluorescence in plant biology (Holland: Kluwer Academic Publishers) pp109-132
[17] Kotzagianni M, Couris S 2013 Chemical Physics Letters 561 36
[18] Xu M F, Shi Y B, Gao W H, Chen H Y 2014 Chinese Journal of Lasers 41 0108005 (in Chinese) [徐美芳, 石云波, 高文宏, 陈海洋 2014 中国激光 41 0108005]
[19] Mukamel E A, Babcock H, Zhuang X W 2012 Biophysical Journal 102 2391
[20] Wan W B, Hua D X, Le J, Liu M X, Cao N 2013 Acta Phys. Sin. 62 190601(in Chinese) [万文博, 华灯鑫, 乐静, 刘美霞, 曹宁 2013 物理学报 62 190601]
[21] Lichtenthaler H K 2011 Applications of chlorophyll fluorescene (Holland: Kluwer Academic Publishers) pp182-196
[22] Kumar P J, Gopal R 2011 Spectroscopy-Biomedical Applications 26 129
[23] Fang W H, Li Z W, Li Z L, Qu G N, Ouyang S L, Men Z W 2012 Acta Phys. Sin. 61 153301(in Chinese) [房文汇, 里佐威, 李占龙, 曲冠男, 欧阳顺利, 门志伟 2012 物理学报 61 153301]
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