Laser micro-spectral analysis coupled with CCD spectrometer was used in this experiment. With Fe Ⅰ356.54 nm and Fe Ⅰ358.12 nm as analysis spectral lines, the micro-plasma temperature and its spatial distribution were investigated in different matrices, namely Mg, Al, Si, and steel alloy 6-0. The electron temperature as a function of location in each matrix and the differences of that at the same location in different matrices were determined and an explanation was given. Finally, with Cu Ⅰ324.75 nm and Zn Ⅰ394.50 nm as analysis spectral lines, we have successfully used the calculated micro-plasma temperature to discuss the matrix effect.