Polarized Raman spectra of single crystal Bi2ZnOB2O6 have been recorded in the spectral range 101600 cm-1 at room temperature. Factor group analysis was used to obtain the normal modes of vibration of the crystal. The Raman peaks under 300 cm-1 are assigned to external modes, which are related to the rotational and transitional movement of the BiO6, ZnO4, BO4 and BO3 groups. Compared with the vibrational spectra of the compounds referred, the satisfactory assignment of most of the high-energy modes to vibrations of Bi-O, B-O and Zn-O bonds can be achieved. In particular, the Raman high-frequency peak located at 1407 cm-1 is attributed to the B-O vibration in the BO3 triangle.