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基于涡旋光照明的暗场数字全息显微方法研究

赵应春 张秀英 袁操今 聂守平 朱竹青 王林 李杨 贡丽萍 冯少彤

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基于涡旋光照明的暗场数字全息显微方法研究

赵应春, 张秀英, 袁操今, 聂守平, 朱竹青, 王林, 李杨, 贡丽萍, 冯少彤

Dark-field digital holographic microscopy by using vortex beam illumination

Zhao Ying-Chun, Zhang Xiu-Ying, Yuan Cao-Jin, Nie Shou-Ping, Zhu Zhu-Qing, Wang Lin, Li Yang, Gong Li-Ping, Feng Shao-Tong
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  • 提出了一种基于涡旋光照明的暗场数字全息显微方法. 从理论上阐述了涡旋光环形照明原理和暗场数字全息显微原理, 分析了涡旋光的准无衍射特性对成像的影响; 搭建了相应的数字全息显微成像系统, 采用690 nm的聚苯乙烯小球作为实验样品; 最后通过对小球明暗场下数字全息显微再现像的分析对比, 证明该方法可以有效地提高数字全息系统的分辨率, 同时增强了再现像的对比度.
    We propose a dark-field digital holographic microscopy (DHM) by using vortex beam illumination. In this paper, the annular illumination of vortex beam and the dark-field DHM imaging system are theoretically analyzed, and the quasi-nondiffracting property of the vortex beam is discussed. A corresponding DHM imaging system is established. The polystyrene spheres each with a size of 690 nm are utilized as objects in the experiment. By comparing the results of reconstructed images under bright-field illumination with those under dark-field illumination DHM, it is proved that the resolution of dark-field DHM under speckle-field illumination is improved and the contrast of its reconstructed image is enhanced accordingly.
    • 基金项目: 国家自然科学基金(批准号:61377003,61275133)、南京师范大学高层次人才科研启动项目(批准号:184080H20162)和南京师范大学青年领军人才培养项目(批准号:184080H20178)资助的课题.
    • Funds: Project supported by the National Natural Science Foundation of China (Grant Nos. 61377003, 61275133), the Scientific Research Foundation for Advanced Talents, Nanjing Normal University, China (Gran No. 184080H20162), and the Training Programme Foundation for Youth Leader Talents by Nanjing Normal University, China (Gran No. 184080H20178).
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    [2]

    Pfeiffer F, Bech M, Bunk O, Kraft P, Eikenberry E F, Brönnimann C, David C 2008 Nat. Mater. 7 134

    [3]

    Verebes G S, Melchiorre M, Garcia-Leis A, Ferreri C, Marzetti C, Torreggiani A 2013 J. Biophoton. 6 960

    [4]

    Dubois F, Grosfils P 2008 Opt. Lett. 33 2605

    [5]

    Faridian A, Pedrini G, Osten W 2013 J. Biomed. Opt. 18 086009

    [6]

    Faridian A, Pedrini G, Osten W 2014 Biomed. Opt. Express 5 728

    [7]

    Park Y, Choi W, Yaqoob Z, Dasari R, Badizadegan K, Feld M S 2009 Opt. Express 17 12285

    [8]

    Zhi S T, Zhang H J, Zhang D X 2012 Acta Phys. Sin. 61 024207 (in Chinese) [支绍韬, 章海军, 张冬仙 2012 物理学报 61 024207]

    [9]

    Li Y Y, Chen Z Y, Liu H, Pu J X 2010 Acta Phys. Sin. 59 1740 (in Chinese) [李阳月, 陈子阳, 刘辉, 蒲继雄 2010 物理学报 59 1740]

    [10]

    Ding P F, Pu J X 2011 Acta Phys. Sin. 60 094204 (in Chinese) [丁攀峰, 蒲继雄 2011 物理学报 60 094204]

    [11]

    Li Z H, Wu F Q, Chen J, Cui X X, Zhu J K 2013 J. Optoelectron Laser 7 1439 (in Chinese) [李志焕, 吴福全, 陈君, 崔祥霞, 朱久凯 2013 光电子激光 7 1439]

    [12]

    Fang G J, Pu J X 2012 Chin. Phys. B 21 084203

    [13]

    Chen Z Y, Pu J X 2012 Chin. Phys. B 21 024201

    [14]

    Porras M A, Borghi R, Santarsiero M 2001 J. Opt. Soc. Am. A 18 177

    [15]

    Born M, Wolf E 2001 Principles of Optics (7th Ed.) (Beijing: World Book Inc) pp467-472

  • [1]

    Villiger M, Pache C, Lasser T 2010 Opt. Lett. 35 3489

    [2]

    Pfeiffer F, Bech M, Bunk O, Kraft P, Eikenberry E F, Brönnimann C, David C 2008 Nat. Mater. 7 134

    [3]

    Verebes G S, Melchiorre M, Garcia-Leis A, Ferreri C, Marzetti C, Torreggiani A 2013 J. Biophoton. 6 960

    [4]

    Dubois F, Grosfils P 2008 Opt. Lett. 33 2605

    [5]

    Faridian A, Pedrini G, Osten W 2013 J. Biomed. Opt. 18 086009

    [6]

    Faridian A, Pedrini G, Osten W 2014 Biomed. Opt. Express 5 728

    [7]

    Park Y, Choi W, Yaqoob Z, Dasari R, Badizadegan K, Feld M S 2009 Opt. Express 17 12285

    [8]

    Zhi S T, Zhang H J, Zhang D X 2012 Acta Phys. Sin. 61 024207 (in Chinese) [支绍韬, 章海军, 张冬仙 2012 物理学报 61 024207]

    [9]

    Li Y Y, Chen Z Y, Liu H, Pu J X 2010 Acta Phys. Sin. 59 1740 (in Chinese) [李阳月, 陈子阳, 刘辉, 蒲继雄 2010 物理学报 59 1740]

    [10]

    Ding P F, Pu J X 2011 Acta Phys. Sin. 60 094204 (in Chinese) [丁攀峰, 蒲继雄 2011 物理学报 60 094204]

    [11]

    Li Z H, Wu F Q, Chen J, Cui X X, Zhu J K 2013 J. Optoelectron Laser 7 1439 (in Chinese) [李志焕, 吴福全, 陈君, 崔祥霞, 朱久凯 2013 光电子激光 7 1439]

    [12]

    Fang G J, Pu J X 2012 Chin. Phys. B 21 084203

    [13]

    Chen Z Y, Pu J X 2012 Chin. Phys. B 21 024201

    [14]

    Porras M A, Borghi R, Santarsiero M 2001 J. Opt. Soc. Am. A 18 177

    [15]

    Born M, Wolf E 2001 Principles of Optics (7th Ed.) (Beijing: World Book Inc) pp467-472

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  • PDF下载量:  1073
  • 被引次数: 0
出版历程
  • 收稿日期:  2014-05-08
  • 修回日期:  2014-06-25
  • 刊出日期:  2014-11-05

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