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基于全量子理论对相干反斯托克斯拉曼散射(CARS)过程进行了分析, 在此基础上搭建了单频CARS显微成像系统, 获得了不同尺寸聚苯乙烯微球高对比度的CARS显微图像. 为了标定成像系统的空间分辨率, 采用逐点扫描方式对直径为110 nm聚苯乙烯微球成像, 从而重构出系统的点扩展函数. 结果表明: 该CARS显微成像系统的横向空间分辨率约为600 nm, 而由阿贝衍射极限决定的理论空间分辨率约为300 nm. 分析了导致分辨率降低的原因, 并提出了解决方案. 为实现纳米分辨的CARS显微成像打下了坚实的基础.
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关键词:
- 相干反斯托克斯拉曼散射 /
- 显微成像 /
- 分辨率
[1] Courjon D, Bainier C 1994 Reports on Progress in Physics 57 989
[2] Axelrod D, Burghardt T P, Thompson N L 1984 Annual Rev. Biophys. Bioengin. 13 247
[3] Hell S W, Wichmann J 1994 Opt. Lett. 19 780
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[11] Heuke S, Legesse F B, Akimov D, Hbner U, Dellith J, Schmitt M, Popp J 2015 JOSA B 32 1773
[12] Hajek K M, Littleton B, Turk D, McIntyre T J, Rubinsztein-Dunlop H 2010 Opt. Express 18 19263
[13] Namboodiri M, Khan T Z, Bom S, Flachenecker G, Materny A 2013 Opt. Express 21 918
[14] Lin J, Er K Z J, Zheng W, Huang Z 2013 Appl. Phys. Lett. 103 083705
[15] Upputuri P K, Wu Z, Gong L, Ong C K, Wang H 2014 Opt. Express 22 12890
[16] Beeker W P, Gro P, Lee C J, Cleff C, Offerhaus H L, Fallnich C, Herek J L, Boller K J 2009 Opt. Express 17 22632
[17] Beeker W P, Lee C J, Boller K J, Gro P, Cleff C, Fallnich C, Offerhaus H L, Herek J L 2010 Phys. Rev. A 81 012507
[18] Beeker W P, Lee C J, Boller K J, Gro P, Cleff C, Fallnich C, Offerhaus H L, Herek J L 2011 J. Raman Spectrosc. 42 1854
[19] Liu W, Niu H B 2011 Phys. Rev. A 83 023830
[20] Liu X, Liu W, Yin J, Qu J L, Lin Z Y, Niu H B 2011 Chin. Phys. Lett. 28 34202
[21] Liu S L, Chen D N, Liu W, Niu H B 2013 Acta Phys. Sin. 62 184210 (in Chinese) [刘双龙, 陈丹妮, 刘伟, 牛憨笨 2013 物理学报 62 184210]
[22] Liu W, Chen D N, Liu S L, Niu H B 2013 Acta Phys. Sin. 62 164202 (in Chinese) [刘伟, 陈丹妮, 刘双龙, 牛憨笨 2013 物理学报 62 164202]
[23] Volkmer A, Book L D, Xie X S 2002 Appl. Phys. Lett. 80 1505
[24] Cheng J X, Book L D, Xie X S 2001 Opt. Lett. 26 1341
[25] Potma E O, Evans C L, Xie X S 2006 Opt. Lett. 31 241
[26] Gachet D, Billard F, Sandeau N, Rigneault H 2007 Opt. Express 15 10408
-
[1] Courjon D, Bainier C 1994 Reports on Progress in Physics 57 989
[2] Axelrod D, Burghardt T P, Thompson N L 1984 Annual Rev. Biophys. Bioengin. 13 247
[3] Hell S W, Wichmann J 1994 Opt. Lett. 19 780
[4] Rust M J, Bates M, Zhuang X 2006 Nature Methods 3 793
[5] Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S 2006 Science 313 1642
[6] Begley R F, Harvey A B, Byer R L 1974 Appl. Phys. Lett. 25 387
[7] Duncan M D, Reintjes J, Manuccia T J 1982 Opt. Lett. 7 350
[8] Zumbusch A, Holtom G R, Xie X S 1999 Phys. Rev. Lett. 82 4142
[9] Cheng J X, Jia Y K, Zheng G F, Xie X S 2002 Biophys. J. 83 502
[10] Lu F, Zheng W, Huang Z 2009 Opt. Lett. 34 1870
[11] Heuke S, Legesse F B, Akimov D, Hbner U, Dellith J, Schmitt M, Popp J 2015 JOSA B 32 1773
[12] Hajek K M, Littleton B, Turk D, McIntyre T J, Rubinsztein-Dunlop H 2010 Opt. Express 18 19263
[13] Namboodiri M, Khan T Z, Bom S, Flachenecker G, Materny A 2013 Opt. Express 21 918
[14] Lin J, Er K Z J, Zheng W, Huang Z 2013 Appl. Phys. Lett. 103 083705
[15] Upputuri P K, Wu Z, Gong L, Ong C K, Wang H 2014 Opt. Express 22 12890
[16] Beeker W P, Gro P, Lee C J, Cleff C, Offerhaus H L, Fallnich C, Herek J L, Boller K J 2009 Opt. Express 17 22632
[17] Beeker W P, Lee C J, Boller K J, Gro P, Cleff C, Fallnich C, Offerhaus H L, Herek J L 2010 Phys. Rev. A 81 012507
[18] Beeker W P, Lee C J, Boller K J, Gro P, Cleff C, Fallnich C, Offerhaus H L, Herek J L 2011 J. Raman Spectrosc. 42 1854
[19] Liu W, Niu H B 2011 Phys. Rev. A 83 023830
[20] Liu X, Liu W, Yin J, Qu J L, Lin Z Y, Niu H B 2011 Chin. Phys. Lett. 28 34202
[21] Liu S L, Chen D N, Liu W, Niu H B 2013 Acta Phys. Sin. 62 184210 (in Chinese) [刘双龙, 陈丹妮, 刘伟, 牛憨笨 2013 物理学报 62 184210]
[22] Liu W, Chen D N, Liu S L, Niu H B 2013 Acta Phys. Sin. 62 164202 (in Chinese) [刘伟, 陈丹妮, 刘双龙, 牛憨笨 2013 物理学报 62 164202]
[23] Volkmer A, Book L D, Xie X S 2002 Appl. Phys. Lett. 80 1505
[24] Cheng J X, Book L D, Xie X S 2001 Opt. Lett. 26 1341
[25] Potma E O, Evans C L, Xie X S 2006 Opt. Lett. 31 241
[26] Gachet D, Billard F, Sandeau N, Rigneault H 2007 Opt. Express 15 10408
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