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超分辨成像及超分辨关联显微技术研究进展

林丹樱 屈军乐

超分辨成像及超分辨关联显微技术研究进展

林丹樱, 屈军乐
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  • 光学成像系统中有限孔径对光波的衍射,使得光学显微成像技术的分辨率受到衍射极限限制而无法进一步提高.自1873年E.K.Abbe提出该问题以来,衍射极限就一直是学术界研究的热点.近年来,随着高强度激光、高灵敏探测器等光电器件研制技术以及新型荧光探针开发等相关领域的快速发展,光学显微技术衍射极限问题的研究迎来了新的契机,超分辨显微成像技术(super-resolution microscopy,SRM)在近十年内取得了令人瞩目的巨大成就.本文从空域和频域角度回顾了衍射极限分辨率的基本原理,并据此对目前常见的各种SRM技术绕过衍射极限提高分辨率的机理给予了详解,同时介绍了各类技术的发展动态和研究方向;作为SRM的一个新的重要的发展趋势,本文详细介绍了超分辨关联显微技术的最新研究进展,包括SRM与活细胞实时荧光显微、荧光寿命显微、光谱测量和成像、电子显微、原子力显微、质谱技术等的关联,着重讨论了各类超分辨关联显微技术的作用和意义;最后,对SRM技术和超分辨关联显微技术的未来发展方向进行了展望.
      通信作者: 屈军乐, jlqu@szu.edu.cn
    • 基金项目: 国家重点基础研究发展计划(批准号:2015CB352005)、国家自然科学基金(批准号:61525503,61378091,61620106016)和广东省自然科学基金创新研究团队项目(批准号:2014A030312008)资助的课题.
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  • [1]

    Volkmann H 1966 Appl. Opt. 5 1720

    [2]

    Airy G B 1835 Trans. Cambridge Philos. Soc. 5 283

    [3]

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    [4]

    Su X Y, Li J T, Cao Y P, Zhang Q C 2011 Information Optics (3rd Ed.) (Beijing: Science Press) p74 (in Chinese) [苏显渝, 李继陶, 曹益平, 张启灿 2011 信息光学(第三版) (北京: 科学出版社) 第74页]

    [5]

    Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S, Davidson M W, Lippincott-Schwartz J, Hess H F 2006 Science 313 1642

    [6]

    Rust M J, Bates M, Zhuang X 2006 Nat. Methods 3 793

    [7]

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    [8]

    Hell S W, Wichmann J 1994 Opt. Lett. 19 780

    [9]

    Klar T A, Jakops S, Dyba M, Hell S W 2000 Proc. Natl. Acad. Sci. USA 97 8206

    [10]

    Gustafsson M G L 2000 J. Microsc. 198 82

    [11]

    Gustafsson M G L 2005 Proc. Natl. Acad. Sci. USA 102 13081

    [12]

    Lin D, Ma W, Liu X, Sun Y, Wu Y 2008 Proc. 2008 CISP 4 244

    [13]

    Thompson R E, Larson D R, Webb W W 2002 Biophys. J. 82 2775

    [14]

    Betzig E 1995 Opt. Lett. 20 237

    [15]

    Dickson R M, Cubitt A B, Tsien R Y, Moerner W E 1997 Nature 388 355

    [16]

    Bates M, Blosser T R, Zhuang X 2005 Phys. Rev. Lett. 94 108101

    [17]

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    [18]

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    [19]

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    [21]

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  • 收稿日期:  2017-04-19
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超分辨成像及超分辨关联显微技术研究进展

  • 1. 深圳大学光电工程学院, 光电子器件与系统教育部/广东省重点实验室, 深圳 518060
  • 通信作者: 屈军乐, jlqu@szu.edu.cn
    基金项目: 

    国家重点基础研究发展计划(批准号:2015CB352005)、国家自然科学基金(批准号:61525503,61378091,61620106016)和广东省自然科学基金创新研究团队项目(批准号:2014A030312008)资助的课题.

摘要: 光学成像系统中有限孔径对光波的衍射,使得光学显微成像技术的分辨率受到衍射极限限制而无法进一步提高.自1873年E.K.Abbe提出该问题以来,衍射极限就一直是学术界研究的热点.近年来,随着高强度激光、高灵敏探测器等光电器件研制技术以及新型荧光探针开发等相关领域的快速发展,光学显微技术衍射极限问题的研究迎来了新的契机,超分辨显微成像技术(super-resolution microscopy,SRM)在近十年内取得了令人瞩目的巨大成就.本文从空域和频域角度回顾了衍射极限分辨率的基本原理,并据此对目前常见的各种SRM技术绕过衍射极限提高分辨率的机理给予了详解,同时介绍了各类技术的发展动态和研究方向;作为SRM的一个新的重要的发展趋势,本文详细介绍了超分辨关联显微技术的最新研究进展,包括SRM与活细胞实时荧光显微、荧光寿命显微、光谱测量和成像、电子显微、原子力显微、质谱技术等的关联,着重讨论了各类超分辨关联显微技术的作用和意义;最后,对SRM技术和超分辨关联显微技术的未来发展方向进行了展望.

English Abstract

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