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Inactivation of Hela cancer cells by an atmospheric pressure cold plasma jet

Huang Jun Chen Wei Li Hui Wang Peng-Ye Yang Si-Ze

Inactivation of Hela cancer cells by an atmospheric pressure cold plasma jet

Huang Jun, Chen Wei, Li Hui, Wang Peng-Ye, Yang Si-Ze
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  • An inactivation mechanism study on Hela cancer cells by means of an atmospheric pressure cold plasma jet is presented. Cell morphology is observed under an inverted microscope after plasma treatment. The neutral red uptake assay provides quantitative evaluations of cell viability under different conditions. The effect of the inactivation efficiency of Hela cancer cells in the argon (900 mL/min) with addition of different amount of oxygen (1%, 2%, 4%, 8%) into atmospheric pressure cold plasma jet is discussed under the fixed power 18 W. Results show that 2% O2 addition provides the best inactivation efficiency, and the survival rate can be reduced to 7% after 180 s treatment. When the oxygen addition exceeds 2%, the inactivation efficiency gradually weakens. The effect is not so good as that in pure argon plasma when the oxygen addition arrives at 8%. According to the emission spectrum of the plasmum, it is concluded that the reactive oxygen species in the plasma play a key role in cancer cell inactivation process.
    • Funds: Project supported by the National Natural Science Foundation of China (Grant Nos. 11275261, 11005151).
    [1]

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    [2]

    Zhang X H, Li M J, Zhou R L, Feng K C, Yang S Z 2008 Appl. Phys. Lett. 93 021502

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    Lee H W, Kim G J, Kim J M, Park J K, Lee J K, Kim G C 2009 Endod. J. 35 587

    [4]

    Chen W, Huang J, Li H, L G H, Wang X Q, Zhang G P, Wang P Y, Yang S Z 2012 Acta Phys. Sin. 61 185203 (in Chinese) [陈维, 黄骏, 李辉, 吕国华, 王兴权, 张国平, 王鹏业, 杨思泽 2012 物理学报 61 185203]

    [5]

    Zhang X H, Huang J, Liu X D, Peng L, Sun Y, Chen W, Feng K C, Yang S Z 2009 Acta Phys. Sin. 58 1595 (in Chinese) [张先徽, 黄骏, 刘筱娣, 彭磊, 孙岳, 陈维, 冯克成, 杨思泽 2009 物理学报 58 1595]

    [6]

    Xiong Z L, Cao Y G, Lu X P, Du T 2011 IEEE Trans. Plasma Sci. 39 2968

    [7]

    Keidar M, Walk R, Shashurin A, Srinivasan P, Sandler A, Dasgupta S, Ravi R, Guerrero-Preston R, Trink B 2011 British Journal of Cancer 105 1295

    [8]

    Huang J, Chen W, Li H, Wang X Q, L G H, Latif Khosa M, Guo M, Feng K C, Wang P Y, Yang S Z 2011 J. Appl. Phys. 109 053305

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    Huang J, Li H, Chen W, L G H, Wang X Q, Zhang G P, Ostrikov K, Wang P Y, Yang S Z 2011 Appl. Phys. Lett. 99 253701

    [10]

    Iseki S, Nakamura K, Hayashi M, Tanaka H, Kondo H, Kajiyama H, Kano H, Kikkawa F, Hori M 2012 Appl. Phys. Lett. 100 113702

    [11]

    Chen W, Huang J, Du N, Liu X D, Wang X Q, L G H, Zhang G P, Guo L H, Yang S Z 2012 J. Appl. Phys. 112 013304

    [12]

    Chen W, Huang J, Du N, Liu X D, L G H, Wang X Q, Zhang G P, Guo L H, Yang S Z 2012 Chin. Phys. Lett. 29 075203

    [13]

    Zhang X H, Huang J, Liu X D, Peng L, Guo L H, L G H, Chen W, Feng K C, Yang S Z 2009 J. Appl. Phys. 105 063302

    [14]

    Chen G L, Zheng X, L G H, Zhang Z X, Sylvain M, Wilson S, Michael T, Yang S Z 2012 Chin. Phys. B 21 105201

    [15]

    Deng S X, Cheng C, Ni G H, Meng Y D, Chen H 2010 Chin. Phys. B 19 105203

    [16]

    Valdespino V M, Valdespino V E 2006 Curr. Opin Obstet Gynecol. 18 35

    [17]

    Yang L, Parkin D M, Ferlay J, Ferlay J, Li L, Chen Y D 2005 Cancer Epidemiology, Biomarkers and Prevention 14 243

    [18]

    Massad L S, Xie X, Darragh T M, Minkoff H, Levine A M, D'Souza G, Cajigas A, Colie C, Watts D H, Strickler H 2009 Obstet Gynecol. 114 1063

    [19]

    Bondar L, Hoogeman M S, Vásquez Osorio E M, Heijmen B J 2010 Med. Phys. 37 3760

    [20]

    Parkin D M, Bray F I, Devesa S S 2001 Eur. J. Cancer 37 S4

    [21]

    Domingues A P, Mota F, Durao M, Frutuoso C, Amaral N, de Oliveira C F 2010 International Journal of Gynecological Cancer 20 294

  • [1]

    Sladek R E J, Stoffels E, Walraven R, Tielbeek P J A, Koolhoven R A 2004 IEEE Trans. Plasma Sci. 32 1540

    [2]

    Zhang X H, Li M J, Zhou R L, Feng K C, Yang S Z 2008 Appl. Phys. Lett. 93 021502

    [3]

    Lee H W, Kim G J, Kim J M, Park J K, Lee J K, Kim G C 2009 Endod. J. 35 587

    [4]

    Chen W, Huang J, Li H, L G H, Wang X Q, Zhang G P, Wang P Y, Yang S Z 2012 Acta Phys. Sin. 61 185203 (in Chinese) [陈维, 黄骏, 李辉, 吕国华, 王兴权, 张国平, 王鹏业, 杨思泽 2012 物理学报 61 185203]

    [5]

    Zhang X H, Huang J, Liu X D, Peng L, Sun Y, Chen W, Feng K C, Yang S Z 2009 Acta Phys. Sin. 58 1595 (in Chinese) [张先徽, 黄骏, 刘筱娣, 彭磊, 孙岳, 陈维, 冯克成, 杨思泽 2009 物理学报 58 1595]

    [6]

    Xiong Z L, Cao Y G, Lu X P, Du T 2011 IEEE Trans. Plasma Sci. 39 2968

    [7]

    Keidar M, Walk R, Shashurin A, Srinivasan P, Sandler A, Dasgupta S, Ravi R, Guerrero-Preston R, Trink B 2011 British Journal of Cancer 105 1295

    [8]

    Huang J, Chen W, Li H, Wang X Q, L G H, Latif Khosa M, Guo M, Feng K C, Wang P Y, Yang S Z 2011 J. Appl. Phys. 109 053305

    [9]

    Huang J, Li H, Chen W, L G H, Wang X Q, Zhang G P, Ostrikov K, Wang P Y, Yang S Z 2011 Appl. Phys. Lett. 99 253701

    [10]

    Iseki S, Nakamura K, Hayashi M, Tanaka H, Kondo H, Kajiyama H, Kano H, Kikkawa F, Hori M 2012 Appl. Phys. Lett. 100 113702

    [11]

    Chen W, Huang J, Du N, Liu X D, Wang X Q, L G H, Zhang G P, Guo L H, Yang S Z 2012 J. Appl. Phys. 112 013304

    [12]

    Chen W, Huang J, Du N, Liu X D, L G H, Wang X Q, Zhang G P, Guo L H, Yang S Z 2012 Chin. Phys. Lett. 29 075203

    [13]

    Zhang X H, Huang J, Liu X D, Peng L, Guo L H, L G H, Chen W, Feng K C, Yang S Z 2009 J. Appl. Phys. 105 063302

    [14]

    Chen G L, Zheng X, L G H, Zhang Z X, Sylvain M, Wilson S, Michael T, Yang S Z 2012 Chin. Phys. B 21 105201

    [15]

    Deng S X, Cheng C, Ni G H, Meng Y D, Chen H 2010 Chin. Phys. B 19 105203

    [16]

    Valdespino V M, Valdespino V E 2006 Curr. Opin Obstet Gynecol. 18 35

    [17]

    Yang L, Parkin D M, Ferlay J, Ferlay J, Li L, Chen Y D 2005 Cancer Epidemiology, Biomarkers and Prevention 14 243

    [18]

    Massad L S, Xie X, Darragh T M, Minkoff H, Levine A M, D'Souza G, Cajigas A, Colie C, Watts D H, Strickler H 2009 Obstet Gynecol. 114 1063

    [19]

    Bondar L, Hoogeman M S, Vásquez Osorio E M, Heijmen B J 2010 Med. Phys. 37 3760

    [20]

    Parkin D M, Bray F I, Devesa S S 2001 Eur. J. Cancer 37 S4

    [21]

    Domingues A P, Mota F, Durao M, Frutuoso C, Amaral N, de Oliveira C F 2010 International Journal of Gynecological Cancer 20 294

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  • Received Date:  22 September 2012
  • Accepted Date:  08 November 2012
  • Published Online:  20 March 2013

Inactivation of Hela cancer cells by an atmospheric pressure cold plasma jet

  • 1. Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Science, Beijing 100190, China;
  • 2. Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, School of Physics and Mechanical and Electrical Engineering, Xiamen University, Xiamen 361005, China
Fund Project:  Project supported by the National Natural Science Foundation of China (Grant Nos. 11275261, 11005151).

Abstract: An inactivation mechanism study on Hela cancer cells by means of an atmospheric pressure cold plasma jet is presented. Cell morphology is observed under an inverted microscope after plasma treatment. The neutral red uptake assay provides quantitative evaluations of cell viability under different conditions. The effect of the inactivation efficiency of Hela cancer cells in the argon (900 mL/min) with addition of different amount of oxygen (1%, 2%, 4%, 8%) into atmospheric pressure cold plasma jet is discussed under the fixed power 18 W. Results show that 2% O2 addition provides the best inactivation efficiency, and the survival rate can be reduced to 7% after 180 s treatment. When the oxygen addition exceeds 2%, the inactivation efficiency gradually weakens. The effect is not so good as that in pure argon plasma when the oxygen addition arrives at 8%. According to the emission spectrum of the plasmum, it is concluded that the reactive oxygen species in the plasma play a key role in cancer cell inactivation process.

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