用斜入射光反射差法无标记实时监测不同浓度兔IgG和山羊抗兔IgG反应的动力学过程

何立平; 孙悦; 戴俊; 王京义; 吕惠宾; 王淑芳; 金奎娟; 周岳亮; 杨国桢

doi:10.7498/aps.61.060703

摘要

用斜入射光反射差法无标记实时监测1.25 mg/mL, 2.50 mg/mL和5.00 mg/mL三种不同浓度兔IgG和浓度为0.02 mg/mL山羊抗兔IgG反应的动态过程, 同时监测和获得三种不同浓度IgG反应的动力学曲线以及相应的反应时间. 实验结果表明, 斜入射光反射差法不仅能无标记地判别生物分子之间是否发生反应, 而且能无标记实时监测生物分子反应的动力学过程, 在生物分子相互作用的研究方面具有潜在和广泛的应用前景.

关键词:

Abstract

We successfully label-free and real-time detecte the interactional dynamic processes of rabbit IgG with different concentrations (1.25 mg/mL, 2.50 mg/mL and 5.00 mg/mL), and goat anti-rabbit IgG with a concentration of 0.02 mg/mL by the oblique-incidence reflectivity difference method (OIRD). The dynamic curves and times of interaction, response to concentration of rabbit IgG, are obtained. The experimental results demonstrate that the OIRD method can not only can distinguish the biomolecular interactions, but also real-time detect the interactional dynamic processes of biomolecules, indicating that the OIRD method has promising and extensive applications for the investigating of biomolecular interactions.

Keywords:

oblique-incidence reflectivity difference method /
label-free and real-time detection /
interaction dynamic process of proteins

作者及机构信息

1.
河北大学物理科学与技术学院, 保定 071002;

2.
中国科学院物理研究所, 北京凝聚态物理国家实验室, 北京 100190

通信作者: 吕惠宾, hblu@iphy.ac.cn ; 王淑芳, hblu@iphy.ac.cn ;

基金项目: 国家重点基础研究发展计划(973计划)(批准号:2007CB935701)资助的课题.

Authors and contacts

1.
College of Physics Science and Technology, Hebei University, Baoding 071002, China;

2.
Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China

Corresponding author: Lü Hui-Bin, hblu@iphy.ac.cn ; Wang Shu-Fang, hblu@iphy.ac.cn ;

Funds: Project supported by the National Basic Research Program of China (Grant No. 2007CB935701).

参考文献

[1]	Zhu H, Bilgin M, Bangham R, Hall D, Casamayor A, Bertone P, Lan N, Jansen R, Bidlingmaier S, Houfek T, itchell T, Miller P, Dean R A, Gerstein M, Snyder M 2001 Science 293 2101
[2]	Espina V,Woodhouse E C, Wulfkuhle J, Asmussen H D, Petricoin E F, Liotta L A 2004 J. Immunol. Methods 290 121
[3]	Yu X B, Xu D K, Cheng Q 2006 Proteomics 6 5493
[4]	Ray S, Mehta G, Srivastava S 2010 Proteomics 10 731
[5]	Han M, Gao X, Su J Z, Nie S 2001 Nat. Biotechnol. 19 631
[6]	Gao X, Cui Y, Levenson R M, Chung L W K, Nie S M 2004 Nat. Biotechnol. 22 969
[7]	Li P Y, Lin B, Gerstenmaier J, Cunningham B T 2004 Sens. Actuat. B 99 6
[8]	Zhu X D, Landry J P, Sun Y S, Gregg J P, Lam K S, Guo X W 2007 Appl. Opt. 46 1890
[9]	Fei Y Y, Landry J P, Sun Y S, Zhu X D, Luo J T,Wang X B, Lam K S 2008 Rev. Sci. Instrum. 79 013708
[10]	Wang X, Yuan K, Lu H, Wen J, Lu H B, Jin K J, Zhou Y L, Yang G Z, Li W, Ruan K C 2010 J. Appl. Phys. 107 063109
[11]	Wang X, Lu H, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2010 Chin. Phys. Lett. 27 107801
[12]	Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Ruan K C, Yang G Z 2010 J. Opt. 12 095301
[13]	Yuan K, Wang X, Lu H, Wen J, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1434
[14]	Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1230
[15]	Wen J, Lu H, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 306
[16]	Wang X, Lu H, Dai J, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2011 Chin. Phys. B 20 010704
[17]	Zhang H Y, Lu H, Li W, Liang R Q, Jin K J, Zhou Y L, Ruan K C, Yang G Z 2008 Chin. Phys. B 17 2288
[18]	Zhu X D, Fei Y Y, Wang X, Lu H B, Yang G Z 2007 Phys. Rev. B 75 245434
[19]	Liang R Q, Tan C Y, Ruan K C 2004 J. Imminol. Methods 285 157

施引文献

[1]	Zhu H, Bilgin M, Bangham R, Hall D, Casamayor A, Bertone P, Lan N, Jansen R, Bidlingmaier S, Houfek T, itchell T, Miller P, Dean R A, Gerstein M, Snyder M 2001 Science 293 2101
[2]	Espina V,Woodhouse E C, Wulfkuhle J, Asmussen H D, Petricoin E F, Liotta L A 2004 J. Immunol. Methods 290 121
[3]	Yu X B, Xu D K, Cheng Q 2006 Proteomics 6 5493
[4]	Ray S, Mehta G, Srivastava S 2010 Proteomics 10 731
[5]	Han M, Gao X, Su J Z, Nie S 2001 Nat. Biotechnol. 19 631
[6]	Gao X, Cui Y, Levenson R M, Chung L W K, Nie S M 2004 Nat. Biotechnol. 22 969
[7]	Li P Y, Lin B, Gerstenmaier J, Cunningham B T 2004 Sens. Actuat. B 99 6
[8]	Zhu X D, Landry J P, Sun Y S, Gregg J P, Lam K S, Guo X W 2007 Appl. Opt. 46 1890
[9]	Fei Y Y, Landry J P, Sun Y S, Zhu X D, Luo J T,Wang X B, Lam K S 2008 Rev. Sci. Instrum. 79 013708
[10]	Wang X, Yuan K, Lu H, Wen J, Lu H B, Jin K J, Zhou Y L, Yang G Z, Li W, Ruan K C 2010 J. Appl. Phys. 107 063109
[11]	Wang X, Lu H, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2010 Chin. Phys. Lett. 27 107801
[12]	Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Ruan K C, Yang G Z 2010 J. Opt. 12 095301
[13]	Yuan K, Wang X, Lu H, Wen J, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1434
[14]	Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1230
[15]	Wen J, Lu H, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 306
[16]	Wang X, Lu H, Dai J, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2011 Chin. Phys. B 20 010704
[17]	Zhang H Y, Lu H, Li W, Liang R Q, Jin K J, Zhou Y L, Ruan K C, Yang G Z 2008 Chin. Phys. B 17 2288
[18]	Zhu X D, Fei Y Y, Wang X, Lu H B, Yang G Z 2007 Phys. Rev. B 75 245434
[19]	Liang R Q, Tan C Y, Ruan K C 2004 J. Imminol. Methods 285 157

计量

文章访问数: 5661
PDF下载量: 512
被引次数: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

搜索

留言板