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THE IMAGING THEORY OF TWO-PHOTON AND MULTI-PHOTON CONFOCAL SCANNING MICROSCOPY

TANG ZHI-LIE LIANG RUI-SHENG CHANG HONG-SEN

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THE IMAGING THEORY OF TWO-PHOTON AND MULTI-PHOTON CONFOCAL SCANNING MICROSCOPY

TANG ZHI-LIE, LIANG RUI-SHENG, CHANG HONG-SEN
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  • The Imaging theory of two-photon and multi-photon confocal scanning microscopy is presented. The three-dimensional point spread function and three-dimensional optical transfer function of two-photon confocal scanning microscopy is derived. By using the three-dimensional point spread function and three-dimensional optical transfer function theory, we have proved that the horizontal and vertical resolutions of two-photon confocal scanning microscopy are higher than those of the single photon confocal scanning microscopy, and the space resolution of multi-photon confocal scanning microscopy is higher than that of two-photon confocal scanning microscopy.
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  • Abstract views:  8337
  • PDF Downloads:  2033
  • Cited By: 0
Publishing process
  • Received Date:  13 October 1999
  • Accepted Date:  18 November 1999
  • Published Online:  05 March 2000

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