In this paper, we report the use of combining holography and photo-induced polymerization techniques to fabricate 2D mesoscale Penrose quasicrystals using 5 coherent beams. The effects of exposure dosage and polarizations of laser beams on the structures are studied. It is demonstrated that the air holes in the 2D quasicrytals could be as small as 0.1μm for high exposure dosage samples. This setup can be used to fabricate versatile 2D mesoscale quasicrystals, and may also shed light on extending this method to 3D quasicrystals. Here we offer a novel method to fabricate quasicrystals which is simpler and cheaper than, if not superior to, the other traditional micro-fabrication methods.