Electron Stimulated Desorption (BSD) of O+ from polycrystal Ni surface was studied. The ESD O+ was measured after the Ni sample was annealed at 700℃ for two hour and then exposed to 20-60 L O2 at room temperature and 500℃ respectively. Two kinds of ESD O+ had been found with different electron energy thresholds (29 eV and 23 eV). It was concluded that the two O+s were due to Auger induced desorption and the two thresholds corresponded to O(2s) electron ionization energy and excitation energy respectively. The process of excitation, ionization and de-excitation was also discussed.