The two-phase structure invariants for the crystal, ψ2=ψH+ψH, are calculated, and the statistical distribution of negative Ψ2 with |E| is obtained. The results for this crystal show that Hauptman's probabilistic formula (1) may reliably determine the signs of Ψ2 as long as their |E| values are not too small in practical use. The computational results for other 5 crystals, C18H24NO4 and its derivatives, also support the above conclusion