The C60+ fragmentation caused by electron collision has been studied with a 911A ion source of a mini-separator at the Accelerator Laboratory of Fudan University. The mass spectrum of the fragments has been measured. We found that the fragments contain even number of carbon atoms and the strength of the beam current shows an exponential distribution. Our results show that the fragmentation caused by electron collision has some similarities to that of atomic collision at the energy about 300 keV.