The surface fluorescence of Rhodamine B (RhB) adsorbed on colloidal silver was investigated. It was found that the 572.5 nm fluorescence band of RhB quenched sharply. However, at the same time the enhancement factor of the other fluorescence band of RhB at 530.0 nm was up to 60 when Ag sol was added.The time-effect fluorescence spectra of RhB adsorbed on Ag surface have been observed by OMA, showing that the quenching and enhancement processes of these two bands were inversed each other at any moment of observation.