Plasma-Polymerized organic film starting from thiophene has been prepared. The elemental composition of pristine film was determined by elastic recoil detection (ERD) and Rutherford backscattering spectrum (RBS). FT-IR technique has been used to characterize the sample film. From the near-IR to UV reflectance and transmittance data, we calculated the optical constants of the film, N(ω) = n + ik ,by using the transfer matrix method. In view of the calculations, we investigated the optical properties of the pristine in detail. Moreover, the doping effect in the plasma-polymerized thiophene film with 24 keV I+ implantation has been also explored. The result shows that the transport process of charge carrier in bombarded layer can be interpreted by Mott's VRH model.