It was shown theoretically and experimentally that a metamaterial composed of periodically positioned split ring resonators (SRR) can lead to a negative effective permeability μeff close to its resonance frequency. In this paper, the nearest neighbor interactions between hexagon SRR were investigated in experiment. The experimental results show that there is only one resonance frequency for a one-dimensional negative permeability μeff material. The main and defect resonance frequencies have a shift when the defect SRR is introduced into the materials, and the shifts of which increase with the resonance frequency difference between defect and based SRR. It is thought that the interaction between SRR becomes stronger when the resonance frequency of the defect SRR is close to that of the based SRR.