Ag/TCNQ (7,7,8,8-tetracyanoquinodimethane) bi-layer films with different thickness were prepared by physical vapor deposition method. The metal-organic complex Ag-TCNQ is formed through transport. The transport behavior in the film was studied using transmission spectrum to monitor the process. Tunneling model and non-tunneling model were established respectively based on whether the tunneling condition was met. Theoretical analysis was carried out in detail. The results of our theoretical models are in good agreement with the experiment data.