Effect of the non-uniform input image on the characteristics of output image for the optical novelty filter based bacteriorhodopsin film was investigated. In contrast to the input image with uniform light distribution， when the incident intensity of the blue and yellow beam changes as the sine function， the light distribution of the output image changes non-monotonically and there are two peaks with weak intensity value between them. The lifetime τ of M state and the velocity of the object also affect the light distribution on the output image. The longer the lifetime τ of M state， the smaller the transmitted intensity of the blue light， and the reduction in intensity decreases with increasing lifetime τ， approaching a value practically equal to zero when τ is longer than 50s. Under the same incident intensity， the intensity of the blue beam at the corresponding position of the output image increases with the increase of velocity of the input image.