The electron impact excitation(EIE) cross sections of He atom and He-like Fe24+, Xe52+ and U90+ ions from the metastable 1s2s3S1 to all of 1snl(n=2,3; l=s,p,d) states are calculated systematically by using the fully relativistic distorted-wave (RDWB) program developed by our group recently. The effects of the Breit interaction on the EIE cross sections of He-like isoelectronic sequences ions at different energies of incident electrons is studied, and some important conclusions are summarized, which can be extended to other highly charged ions. Also, comparison of the present calculations for 1s2s3S1→1s2p3P and 1s2s3S1→1s3s3S1 with the available experimental results gave good agreement.