Serials of spin ladder compound Sr14(Cu1-yFey)24O41 with different doping level have been prepared by conventional solid state reaction method. The polarized Raman spectroscopy shows the existence of (4Ag+4B1g+3B2g+B3g) modes， and the peak at 1170 cm-1 for all the samples are associated with the longitudinal phonon mode which reveals the information of the ladder. Furthermore， we have compared the result of the Raman spectroscopy with the conduction behavior.