Most recently, both BABAR and Belle experiments found evidences of neutral D mixing. In this paper, we discuss the constraints on the strong phase difference in D0→Kπ decay from the measurements of the mixing parameters y′, yCP, and x at the B-factories. With CP tag technique at ψ（3770） peak, the extraction of the strong phase difference at BES-Ⅲ is discussed. The sensitivity of the measurement of the mixing parameter y is estimated in BES-Ⅲ experiment at ψ（3770） peak. Finally, we make an estimate on the measurements of mixing rate RM.