The effect of spontaneously generated coherence （SGC） on transient evolution of gain of lasing without inversion （LWI） and modulation roles of the injection rate ratio and exit rate on SGC-dependent gain in an open Ladder type three level system are studied by using the numerical solution of the density matrix motion equation of the system. It was shown that: variation of SGC strength will make the transient and final stationary gains having a considerable changing; when incoherent pumping presents, LWI gain increases with the ratio of injection rates and exit rate decreasing, however, when incoherent pumping is absent, the case is just the opposite; by choosing suitable values of SGC strength, the injection rate ratio and exit rate, we can obtain the largest transient and final stationary gain.