Close-coupling method was applied to the 3He（4He,5He）-H2 system, and the second vibrational excitation cross sections of “00-20，00-22，00-24，00-26” at different incident energies have been calculated．By analysing the differences of these partial wave cross sections, the change rules of how the partial wave cross sections change with the mass of isotope atom and the relative kinetic energy of incident atoms are obtained.