The anti-collision theory of adaptive Q algorithm and radio frequency identification(RFID) communication timing sequence were analyzed. Then a Markov chain model for simulating multi-tag identification process was established based on the following three definitions. The definitions are identifying efficiency, identifying speed and data state(Q, n), where n is the total remanent tag number, and Q is the slot count. The identifying efficiency and speed were obtained from the model using Monte Carlo statistical method. A software-defined radio program was built to simulate the collision of RFID which could quantify the identifying efficiency and speed effectively. The consistency of model simulation result and test data proves the validity of the model and test method.