In this paper, we investigate the joint production of charged top-pions pair and Z gauge boson in the topcolor-assisted technicolor (TC2) at the International Linear Collider (ILC) via the process e+e- → Zπt+πt-. We calculate the production cross section of the process e+e- → Zπt+πt- and find that the cross section of the process e+e- → Zπt+πt- can reach 1 fb in a reasonable parameter space of the TC2 model. Considering the main decay mode of charged top-pion πt+ → tb, we find that such a production may provide copious events at the ILC.