Due to the fact that regularization method can overcome the ill-posed characteristic and obviously suppress noise and errors propagation in practical inverse problem, a new algorithm (genetic algorithm with regularization method) is proposed, combining fitness function of genetic algorithm with regularization term to retrieve ocean duct parameter. Simulation experiments show that retrieval accuracy of the new algorithm is better than that of traditional genetic algorithm. When the noise errors are less than 10%, the noise-immune performance is better. Finally, the inversion result from airborne radar clutter local date of sea surface in the Wallops island is compared with real refractivity profile, pointing out the efficiency of algorithm. The new method provides a new consideration for inverse research of ocean atmosphere duct.