The characteristic X-ray spectra produced by the impact of highly charged ions of 152Eu20+ with energies from 2.0 to 6.0 MeV on Au surface are measured. It is found that highly charged ions could excite both the characteristic X-ray spectra of Mζ, Mα and Mδ of Au and the characteristic X-ray spectra of Mα of Eu. The total X-ray yield increases with the ion kinetic energy increasing. The total production cross section of Au induced by Eu20+ is measured and compared with those obtained from the binary encounter approximation, plane-wave-Born approximation, and the energy-loss Coulomb deflection perturbed stationary state relativistic theoretical models.