The conformal invariance and Hojman conserved quantity of Lie symmetry for Appell equations in a holonomic system are studied. Under the special infinitesimal transformations in which the time is not variable, the Lie symmetry and conformal invariance of differential equations of motion for a holonomic system are defined, and the determining equations of the conformal invariance of Lie symmetry and the Hojman conserved quantity for the system are given. Finally, an example is presented to illustrate the application of the results.