Genetic mutant bacteriorhodopsin BR_D96N film was experimentally studied on its holographic storage properties with different polarization recording waves. The influences of the different polarization states of the recording and readout wav es on the retrieval diffractive image's intensity and signal_to_noise_ratio were compared with each other. The experimental results showed that compared with ot her polarization holographic recording, orthogonal circular polarization recordi ng can realize the separation of the polarization state of the diffractive wave from the scattering noise, and thus has high signal_to_noise_ratio as well as hi gh diffraction efficiency. Using He_Ne laser (633nm, 3mW) as recording and reado ut beam, spatial light modulator as data input element and CCD as data capture e lement, orthogonal circular polarization holographic optical data storage was ma de on 60μm×42μm area of the BR_D96N film by Fourier transform holographic met hod. The area density of 2×108bit/cm2 was obtained, and t he encoded data was retrieved without errors.