优化的数字全息显微成像系统

王华英; 刘飞飞; 廖薇; 宋修法; 于梦杰; 刘佐强

doi:10.7498/aps.62.054208

摘要

基于预放大数字全息显微系统的全息图记录与再现过程及其点扩散函数的分析, 从成像分辨率、成像质量及实现的难易程度等方面对数字全息显微中六种常见记录光路系统的成像性能进行了对比研究. 结果表明, 像面数字全息术具有最高的成像分辨率及成像质量, 其成像分辨率与记录器件的光敏面尺寸无关, 该系统对信息的记录是完整的, 而且记录过程不必考虑物体被照亮区域的大小, 再现过程非常简单, 是优化的数字全息显微成像系统. 等波面弯曲的物参光像面数字全息术非常有利于位相解包裹及位相畸变补偿的正确进行, 该系统更适合于位相显微. 实验结果验证了理论分析的正确性.

关键词:

Abstract

Based on the analysis of the recording and reconstructing of hologram and the point spread function of pre-magnification digital micro-holographic system (PMDMHS), the performances of the six common PMDMHSs are compared in imaging resolution, imaging quality and conveniences, for the first time to our knowledge. The results show that the digital image-plane holography (DIPH) has the highest imaging resolution and the best imaging quality; the resolution of DIPH is independent of the photosensitive surface size of the recording device; this system can completely record the information about the object transmitted through the micro-objective (MO); it is not needed to consider the size of illuminated region of object. Moreover, the reconstruction process of DIPH is very simple. DIPH is an optimized digital holographic imaging system. The DIPH with equal curvature of reference wavefront and object wavefront is very conducive to phase unwrapping and phase aberration compensation; therefore DIPH is more suitable for phase microscopy. The experimental results demonstrate the validation of the theoretical analysis.

Keywords:

作者及机构信息

1.
河北工程大学信息与电子工程学院, 邯郸 056038;

2.
河北工程大学理学院, 邯郸 056038

基金项目: 国家自然科学基金(批准号: 61077001和61144005)、河北省自然科学基金(批准号: F2010001038、F2012402028和F2012402051)、河北省科技支撑计划(批准号: 09277101D)和河北省教育厅科技计划重点项目(批准号: ZH2011241)资助的课题.

Authors and contacts

1.
Scholl of Information & Electrical Engineering, Hebei University of Engineering, Handan 056038 China;

2.
College of Science, Hebei University of Engineering, Handan 056038, China

Funds: Project supported by the National Natural Science Foundation of China (Grant Nos. 61077001 and 61144005), the Natural Science Foundation of Hebei Province, China (Grant Nos. F200810001038、F2012402028 and F2012402051), the Key Technology Research and Development Program of Hebei Province (Grant No. 09277101D), and the Scientific Research Fund of Education Department of Hebei Province (Grant No. ZH2011241).

参考文献

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[2]	Anand A, Chhaniwal V K, Javidi B 2010 J. Dis. Tech. 6 500
[3]	Miccio L, Finizio A, Puglisi R 2011 Bio.Opt. Exp. 2 331
[4]	Rajshekhar G, Gorthi S S, Rastogi P 2010 Opt. Exp. 18 18041
[5]	Das B, Yelleswarapu C S, Rao D V G L N 2012 Appl. Opt. 51 1387
[6]	Wang L, Zhao J L, Di J L, Jiang H Z 2011 Opt. Lett. 36 2710
[7]	Wang Y X, Wang D Y, Zhao J, Yang Y S, Xiao X Q, Cui H Q 2011 Chi. Opt. Lett. 9 030901
[8]	Ding S H, Li Q, Li Y D, Wang Q 2011 Opt. Lett. 36 1993
[9]	Zhao J L, Jiang H Z, Di J L 2012 Biomedical Optics and 3D Imaging OSA DSu3C 1
[10]	Tsang P W M, Cheung K W K, Poon T C 2012 Opt. Expre. 20 A5962
[11]	Li J C, Fan Z B, Patrice T, Song Q H, Pascal P 2011 Acta. Phys. Sin. 60 034204 (in Chinese) [李俊昌, 樊则宾, Patrice T, 宋庆和, Pascal P 2011 物理学报 60 034204]
[12]	Hu H F, Wang X L, Li Z L, Zhang N, Zhai H C 2009 Acta. Phys. Sin. 58 7662 (in Chinese) [胡浩丰, 王晓雷, 李智磊, 张楠, 翟宏琛 2009 物理学报 58 7662]
[13]	Hu H F, Wang X L, Gguo W G, Zhai H C, Wang P 2011 Acta. Phys. Sin. 60 017901 (in Chinese) [胡浩丰, 王晓雷, 郭文刚, 翟宏琛, 王攀 2011 物理学报 60 017901]
[14]	Zhang Q S, Lv X Xu, Yu Q T, Liu G Y 2009 Chin. Phy. B 18 2764
[15]	Wang H Y, Guo Z J, Zhang Z H, Liu F F 2012 J. Optoelectronics Laser 39 0209002 (in Chinese) [王华英, 郭中甲, 张志会, 刘飞飞 2012 中国激光 39 0209002]
[16]	Wang H Y, Wang G J, Zhao J, Xie J J, Wang D Y 2008 Acta Photonica Sinica 37 729 (in Chinese) [王华英, 王广俊, 赵洁, 谢建军, 王大勇 2008 光子学报 37 729]
[17]	Cui H K, Wang D Y, Wang Y X, Liu C G, Zhao J, Li Y 2011 Acta. Phys. Sin. 60 044201 (in Chinese) [崔华坤, 王大勇, 王云新, 刘长庚, 赵洁, 李艳 2011 物理学报 60 044201]

施引文献

[1]	Eilenberger F, Minardi S, Pliakis D, Pertsch T 2012 Opt. Lett. 37 509
[2]	Anand A, Chhaniwal V K, Javidi B 2010 J. Dis. Tech. 6 500
[3]	Miccio L, Finizio A, Puglisi R 2011 Bio.Opt. Exp. 2 331
[4]	Rajshekhar G, Gorthi S S, Rastogi P 2010 Opt. Exp. 18 18041
[5]	Das B, Yelleswarapu C S, Rao D V G L N 2012 Appl. Opt. 51 1387
[6]	Wang L, Zhao J L, Di J L, Jiang H Z 2011 Opt. Lett. 36 2710
[7]	Wang Y X, Wang D Y, Zhao J, Yang Y S, Xiao X Q, Cui H Q 2011 Chi. Opt. Lett. 9 030901
[8]	Ding S H, Li Q, Li Y D, Wang Q 2011 Opt. Lett. 36 1993
[9]	Zhao J L, Jiang H Z, Di J L 2012 Biomedical Optics and 3D Imaging OSA DSu3C 1
[10]	Tsang P W M, Cheung K W K, Poon T C 2012 Opt. Expre. 20 A5962
[11]	Li J C, Fan Z B, Patrice T, Song Q H, Pascal P 2011 Acta. Phys. Sin. 60 034204 (in Chinese) [李俊昌, 樊则宾, Patrice T, 宋庆和, Pascal P 2011 物理学报 60 034204]
[12]	Hu H F, Wang X L, Li Z L, Zhang N, Zhai H C 2009 Acta. Phys. Sin. 58 7662 (in Chinese) [胡浩丰, 王晓雷, 李智磊, 张楠, 翟宏琛 2009 物理学报 58 7662]
[13]	Hu H F, Wang X L, Gguo W G, Zhai H C, Wang P 2011 Acta. Phys. Sin. 60 017901 (in Chinese) [胡浩丰, 王晓雷, 郭文刚, 翟宏琛, 王攀 2011 物理学报 60 017901]
[14]	Zhang Q S, Lv X Xu, Yu Q T, Liu G Y 2009 Chin. Phy. B 18 2764
[15]	Wang H Y, Guo Z J, Zhang Z H, Liu F F 2012 J. Optoelectronics Laser 39 0209002 (in Chinese) [王华英, 郭中甲, 张志会, 刘飞飞 2012 中国激光 39 0209002]
[16]	Wang H Y, Wang G J, Zhao J, Xie J J, Wang D Y 2008 Acta Photonica Sinica 37 729 (in Chinese) [王华英, 王广俊, 赵洁, 谢建军, 王大勇 2008 光子学报 37 729]
[17]	Cui H K, Wang D Y, Wang Y X, Liu C G, Zhao J, Li Y 2011 Acta. Phys. Sin. 60 044201 (in Chinese) [崔华坤, 王大勇, 王云新, 刘长庚, 赵洁, 李艳 2011 物理学报 60 044201]

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