Light-sheet fluorescence microscopy (LSFM) uses a thin light sheet to excite the specimen from the side and perform imaging in the direction perpendicular to the light-sheet. It has the advantages of fast imaging speed, high optical sectioning ability and low photobleaching and phototoxicity to samples. Therefore, it is suitable for high-quality, long-term three-dimensional dynamic observation of large living biological samples. However, the traditional Gaussian light sheet illumination microscopy technology has the problems of small imaging field of view and low spatial resolution. Based on the existing dual-sided illumination LSFM, a large field of view and high resolution LSFM combined with virtual single-pixel imaging deconvolution is presented in this paper, which improves simultaneously the field of view and resolution of LSFM. The related microscope was designed and built, and three-dimensional optical sectioning imaging experiments of fluorescent beads and transgenic zebrafish standard samples were carried out. The experimental results prove the three-dimensional high resolution imaging capability of the microscope, which is of great significance for the development of large field of view and high resolution LSFM.