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提出和发展了基于毛细管-微球组合探针的任选区、 高分辨显微成像新方法. 建立了微球显微成像的物理模型, 利用成像理论,推导出微球成像的放大倍率; 采用3.0, 4.4, 5.6, 7.5, 10.0 μm等不同直径的SiO2微球, 对未经刻录的DVD光盘进行了微球显微成像实验, 可以观察到DVD光盘的微纳米结构被明显放大且对比度显著提高, 与理论计算结果相符合; 采用毛细管微探针操纵微球的方法, 实现了基于微球透镜阵列的样品微纳米结构的高分辨显微成像; 在此基础上, 进一步将毛细管微探针与微球组合, 制备出毛细管-微球组合型探针, 首次实现了基于微球透镜的样品任意区域高分辨显微成像.
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关键词:
- 微球 /
- 显微成像 /
- 毛细管-微球组合探针 /
- 高分辨
In this paper, we propose a novel location-free high-resolution optical microscopic imaging method based on micropipette-microsphere combined probe. According to optical principle, a physical model is established to determine the value of magnification. With SiO2 microspheres of different diameters such as 3.0, 4.4, 5.6, 7.5 and 10.0 μm, microscopic imaging experiments are carried out. The results show that the microstructure of un-etched DVD disk can be significantly magnified and the image contrast is obviously improved, which is consistent well with the theoretical calculation. Microscopic imaging of microstructures based on SiO2 microsphere array is realized by controlling the microspheres with a micropipette. Furthermore, a kind of micropipette-microsphere combined probe is prepared, by which location-free high-resolution optical microscopic imaging has implemented for the first time.-
Keywords:
- microsphere /
- microscopic imaging /
- micropipette-microsphere combined probe /
- high-resolution
[1] Coene W, Janssen G 1992 Phys. Rev. Lett. 69 3743
[2] Reimer L 2000 Scanning Electron Microscopy (Berlin: Springer-Verlag) p12
[3] Binnig G, Rohrer H 1982 Helev Phys. Acta 55 726
[4] Binnig G, Quate C F, Gerber C 1986 Phys. Rev. Lett. 56 930
[5] Betzig E, Trautman J K 1992 Science 257 189
[6] Ziela R, Hausa A, Tulkeb A 2008 J. Membrane Sci. 323 241
[7] Xiao B, Feng J, Chen J C, Yan J K, Gan G Y 2008 Acta Phys. Sin. 57 3769 (in Chinese) [肖冰, 冯晶, 陈敬超, 严继康, 甘国友 2008 物理学报 57 3769]
[8] Shi B, Zhang H J, Wu L, Zhang D X 2012 Spectrosc. Spect. Anal. 32 993 (in Chinese) [史斌, 章海军, 吴兰, 张冬仙 2012 光谱学与光谱分析 32 993]
[9] Fu X, Zhang H J, Zhang D X 2011 Microsc. Res. Techniq. 74 1058
[10] Wang Z Y, Li Q, Zhao J, Guo J H 2000 Acta Phys. Sin. 49 1959 (in Chinese) [王子洋, 李勤, 赵钧, 郭继华 2000 物理学报 49 1959]
[11] Melville D O S, Blaikie R J 2005 Opt. Express 13 2127
[12] Zhi S T, Zhang H J, Zhang D X 2011 Acta Phys. Sin. 61 024207 (in Chinese) [支绍韬, 章海军, 张冬仙 2011 物理学报 61 024207]
[13] Wang Z B, Guo W, Li L, Boris L Y C, Khan A, Liu Z, Chen Z, Hong M 2011 Nat. Commun. 2 218
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[1] Coene W, Janssen G 1992 Phys. Rev. Lett. 69 3743
[2] Reimer L 2000 Scanning Electron Microscopy (Berlin: Springer-Verlag) p12
[3] Binnig G, Rohrer H 1982 Helev Phys. Acta 55 726
[4] Binnig G, Quate C F, Gerber C 1986 Phys. Rev. Lett. 56 930
[5] Betzig E, Trautman J K 1992 Science 257 189
[6] Ziela R, Hausa A, Tulkeb A 2008 J. Membrane Sci. 323 241
[7] Xiao B, Feng J, Chen J C, Yan J K, Gan G Y 2008 Acta Phys. Sin. 57 3769 (in Chinese) [肖冰, 冯晶, 陈敬超, 严继康, 甘国友 2008 物理学报 57 3769]
[8] Shi B, Zhang H J, Wu L, Zhang D X 2012 Spectrosc. Spect. Anal. 32 993 (in Chinese) [史斌, 章海军, 吴兰, 张冬仙 2012 光谱学与光谱分析 32 993]
[9] Fu X, Zhang H J, Zhang D X 2011 Microsc. Res. Techniq. 74 1058
[10] Wang Z Y, Li Q, Zhao J, Guo J H 2000 Acta Phys. Sin. 49 1959 (in Chinese) [王子洋, 李勤, 赵钧, 郭继华 2000 物理学报 49 1959]
[11] Melville D O S, Blaikie R J 2005 Opt. Express 13 2127
[12] Zhi S T, Zhang H J, Zhang D X 2011 Acta Phys. Sin. 61 024207 (in Chinese) [支绍韬, 章海军, 张冬仙 2011 物理学报 61 024207]
[13] Wang Z B, Guo W, Li L, Boris L Y C, Khan A, Liu Z, Chen Z, Hong M 2011 Nat. Commun. 2 218
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