The fluorescence and up-conversion spectrum of Er3+/Yb3+, Tm3+/Yb3+, Er3+/Yb3+/Tm3+-codoped tellurite glasses pumped by a 970nm laser diode has been studied. The lifetimes of Er3+:4I11/2 and Er3+: 4I13/2 levels have been measured. It is found that the fluorescence width in the range of 1450—1700nm in Er3+/Yb3+/Tm3+-codoped tellurite glass has been broadened clearly. There is a fluorescence peak at 1630nm which is probably due to Tm3+:1G4→3F2 transitions in Er3+/Yb3+/Tm3+-codoped tellurite glass. Up-conversion phenomena exist in all the three kinds coped tellurite glasses because of its lower phonon energy of host. The addition of Tm3+ in Er3+/Yb3+-codoped tellurite glasses quenches the up-conversion efficiency of Er3+:4S3/2 green and Er3+:4F9/2 red emissions. The quenching effect is due to the energy transfer between Er3+ and Tm3+, especially the cross-relaxation process among them.