A review on bioelectrical effects of cellular organelles by high voltage nanosecond pulsed electric fields

Guo Yu-Yi; Shi Fu-Kun; Wang Qun; Ji Zhen-Yu; Zhuang Jie

doi:10.7498/aps.71.20211850

Abstract

The biomedical application of high-voltage nanosecond pulsed electric fields (nsPEFs) has become an emerging interdisciplinary research field in recent years. Compared with microsecond and millisecond pulsed electric fields, high-voltage nsPEFs can not only lead the cell membrane structure to polarize and dielectric break down the cell membrane structure, i.e. membrane electroporation, but also penetrate into the cell, triggering off organelle bioelectrical effects such as cytoskeleton depolymerization, intracellular calcium ion release, and mitochondrial membrane potential dissipation. Extensive attention has been attracted from related academic communities. In this article, the following aspects are involved. First, the physical model of high-voltage nsPEFs and its bioelectrical effects on cellular organelles are introduced. Then, the existing researches of the interactions of high-voltage nsPEFs with cytoskeleton, mitochondria, endoplasmic reticulum, cell nucleus and other subcellular structure are reviewed and summarized; the relationship between the influence on cellular organelles by high-voltage nsPEFs and the biological effects such as cell death and intercellular communication is highlighted. Finally, the key technical challenges to high-voltage nsPEFs in biomedical research are condensed, followed by the prospects of future research directions.

Keywords:

Authors and contacts

1.
Division of Life Sciences and Medicine, School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Suzhou 215000, China
2.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215000, China
3.
Faculty of Military Biomedical Engineering, Air Force Military Medical University, Xi’an 710032, China

Corresponding author: Zhuang Jie, jzhuang@sibet.ac.cn

Funds: Project supported by the National Key R&D Program of China (Grant Nos. 2019YFC0119102, 2019YFC0118004, 2020YFC0122301) .

FullText HTML : translate this paragraph

References

[1]	高宇, 门业堃, 张建, 刘范嘉, 邓永迪, 王振 2016 高电压技术 42 2675 Google Scholar Gao Y, Men Y K, Zhang J, Liu F J, Deng Y D, Wang Z 2016 High Voltage Eng. 42 2675 Google Scholar
[2]	Amr A G, Schoenbch K H 2000 IEEE Trans. Plasma Sci. 28 115 Google Scholar
[3]	Sitzmann W, Vorobiev E, Lebovka N 2016 Innovative Food Sci. Emerg. 37 302 Google Scholar
[4]	Barbosa-Cánovas G V, Altunakar B 2006 Pulsed Electric Fields Technology for the Food Industry: Fundamentals and Applications (Boston: Springer US) pp3–26
[5]	Takaki K, Yoshida K, Saito T, Kusaka T, Yamaguchi R, Takahashi K, Sakamoto Y 2014 Microorganisms 2 58 Google Scholar
[6]	Takaki K, Yamazaki N, Mukaigawa S, Fujiwara T, Kofujita H, Takahasi K, Narimatsu M, Nagane K 2009 Acta Phys. Pol. A 115 1062 Google Scholar
[7]	Deore A B, Sapakal V D, Shikalgar T S, Jagtap M J, Bhinde C J 2017 Innovative Pharm. 5 147
[8]	Heller R, Jaroszeski M J, Reintgen D S, Puleo C A, DeConti R C, Gilbert R A, Glass L F 1998 Cancers 83 148 Google Scholar
[9]	Heller R, Gilbert R, Jaroszeski M J 1999 Adv. Drug Deliver. Rev. 35 119 Google Scholar
[10]	姚陈果, 宁郡怡, 刘红梅, 郑爽, 董守龙 2020 电工技术学报 35 115 Google Scholar Yao C G, Ning J Y, Liu H M, Zheng S, Dong S L 2020 Trans. China Electrotech. Soc. 35 115 Google Scholar
[11]	Golberg A, Broelsch G, Bohr S, Mihm M, Austen W, Albadawi H, Watkins M, Yarmush M 2013 Technology 1 1 Google Scholar
[12]	Golberg A, Yarmush M 2013 IEEE Trans. Bio-Med. Eng. 60 707 Google Scholar
[13]	Schoenbach K H, Joshi R P, Kolb J F, Nianyong C, Stacey M, Blackmore P F, Buescher E S, Beebe S J 2004 Proc. IEEE 92 1122 Google Scholar
[14]	Graybill P M, Davalos R V 2020 Cancers 12 1132 Google Scholar
[15]	Scarlett S S, White J A, Blackmore P F, Schoenbach K H, Kolb J F 2009 Biochim. Biophys. Acta Biomembr. 1788 1168 Google Scholar
[16]	Rubinsky B, Romeo S, Zeni L, Sarti M, Sannino A, Scarfì M R, Vernier P T, Zeni O 2011 PLoS One 6 e28419 Google Scholar
[17]	Beebe S, Sain N, Ren W 2013 Cells 2 136 Google Scholar
[18]	Chen X, Kolb J F, Swanson R J, Schoenbach K H, Beebe S J 2010 Pigm. Cell Melanoma R. 23 554 Google Scholar
[19]	Vadlamani R A, Nie Y, Detwiler D A, Dhanabal A, Kraft A M, Kuang S, Gavin T P, Garner A L 2019 J. R. Soc. Interface 16 20190079 Google Scholar
[20]	Chen J, Huang Y, Yang J, Li K, Jiang Y, Heng B C, Cai Q, Zhang J, Ge Z 2020 J. Tissue Eng. Regen. Med. 14 1136 Google Scholar
[21]	Kim V, Gudvangen E, Kondratiev O, Redondo L M, Xiao S, Pakhomov A 2021 Int. J. Mol. Sci. 22 7051 Google Scholar
[22]	Casciola M, Xiao S, Pakhomov A 2017 Sci. Rep. 7 10453
[23]	Tolstykh G P, Valdez C M, Montgomery N D, Cantu J C, Sedelnikova A, Ibey B L 2021 Bioelectrochemistry 142 107930 Google Scholar
[24]	Stampfli R 1958 An Acad. Brasil Ciens. 30 57
[25]	Neumann E, Schaeferridder M, Wang Y, Hofschneider P H 1982 EMBO. J. 1 841 Google Scholar
[26]	Weaver J C 1993 J. Cell. Biochem. 51 426 Google Scholar
[27]	Glaser R W, Leikin S L, Chernomordik L V, Pastushenko V F, Sokirko A I 1988 Biochim. Biophys. Acta Biomembr. 940 275 Google Scholar
[28]	Weaver J C, Chizmadzhev Y A 1996 Bioelectrochemistry 41 135
[29]	Somiari S, Glasspool-Malone J, Drabick J J, Gilbert R A, Heller R, Jaroszeski M J, Malone R W 2000 Mol. Ther. 2 178 Google Scholar
[30]	史秋生, 杜联芳, 张焱锋 2018 肿瘤影像学 27 242 Shi Q S, Du L F, Zhang Y F 2018 Oncoradiology 27 242
[31]	李小曼, 万利杰, 王晓娟, 谢红梅, 兰建, 刘晓云 2017 现代生物医学进展 17 1190 Google Scholar Li X M, Wan L J, Wang X J, Xie H M, Lan J, Liu X Y 2017 Prog. Mod. Biomed. 17 1190 Google Scholar
[32]	Deipolyi A R, Golberg A, Yarmush M L, Arellano R S, Oklu R 2014 Diagn. Interv. Radiol. 20 147 Google Scholar
[33]	庞小峰 2008 生物电磁学 (北京: 国防工业出版社) 第101页 Pang X F 2008 Bioelectromagnetism (Beijing: National Defense Industry Press) p101 (in Chinese)
[34]	Buescher E S, Schoenbach K H 2003 IEEE Trans. Dielectr. Electr. Insul. 10 788 Google Scholar
[35]	Koga T, Morotomi-Yano K, Sakugawa T, Saitoh H, Yano K I 2019 Sci. Rep. 9 8451 Google Scholar
[36]	Guerrero-Hernandez A, Beebe S J, Chen Y J, Sain N M, Schoenbach K H, Xiao S 2012 PLoS One 7 e51349 Google Scholar
[37]	Napotnik T B, Wu Y H, Gundersen M A, Miklavčič D, Vernier P T 2012 Bioelectromagnetics 33 257 Google Scholar
[38]	Jansen E D, Thomas R J, Wilmink G J, Ibey B L, Estlack L E, Roth C C, Cerna C Z, Wilmink G J, Ibey B L 2014 Proceedings of the SPIE San Francisco, California, USA, March 13, 2014 p89411S
[39]	Rossi A, Pakhomova O N, Mollica P A, Casciola M, Mangalanathan U, Pakhomov A G, Muratori C 2019 Cancers 11 2034 Google Scholar
[40]	Hirai H, Miyake M, Nagano A, Teranishi K, Shimomura N, Oyadomari S 2014 IEEE International Power Modulator and High Voltage Conference (IPMHVC) Santa Fe, New Mexico, USA, June 1–5, 2014 p419
[41]	Thompson G L, Roth C C, Dalzell D R, Kuipers M A, Ibey B L 2014 J. Biomed. Opt. 19 1 Google Scholar
[42]	Thompson G, Beier H, Ibey B 2018 Bioengineering 5 103 Google Scholar
[43]	Schoenbach K H, Katsuki S, Stark R H, Buescher E S, Beebe S J 2002 IEEE Trans. Plasma Sci. 30 293 Google Scholar
[44]	Schoenbach K H 2018 Bioelectromagnetics 39 257 Google Scholar
[45]	姚陈果, 莫登斌, 孙才新, 陈新, 熊正爱 2008 生物医学工程学杂志 25 1206 Google Scholar Yao C G, Mo D B, Sun C X, Chen X, Xiong Z A 2008 J. Biomed. Eng. 25 1206 Google Scholar
[46]	米彦 2009 博士学位论文 (重庆: 重庆大学) Mi Y 2009 Ph. D. Dissertation (Chongqing: Chongqing University) (in Chinese)
[47]	Pakhomov A G, Shevin R, White J A, Kolb J F, Pakhomova O N, Joshi R P, Schoenbach K H 2007 Arch. Biochem. Biophys. 465 109 Google Scholar
[48]	Berghöfer T, Eing C, Flickinger B, Hohenberger P, Wegner L H, Frey W, Nick P 2009 Biochem. Biophys. Res. Commun. 387 590 Google Scholar
[49]	Stacey M, Fox P, Buescher S, Kolb J 2011 Bioelectrochemistry 82 131 Google Scholar
[50]	Pakhomov A G, Xiao S, Pakhomova O N, Semenov I, Kuipers M A, Ibey B L 2014 Bioelectrochemistry 100 88 Google Scholar
[51]	Wilmink G J, Thompson G L, Roth C, Tolstykh G, Kuipers M, Ibey B L, Ibey B L 2013 Proceedings of the SPIE San Francisco, California, USA, February 23, 2013 p85850T
[52]	Steuer A, Schmidt A, Labohá P, Babica P, Kolb J F 2016 Bioelectrochemistry 112 33 Google Scholar
[53]	Carr L, Bardet S M, Burke R C, Arnaud-Cormos D, Leveque P, O'Connor R P 2017 Sci. Rep. 7 41267
[54]	Tolstykh G P, Thompson G L, Beier H T, Steelman Z A, Ibey B L 2017 BB Reports 9 36 Google Scholar
[55]	Perrier D L, Vahid A, Kathavi V, Stam L, Rems L, Mulla Y, Muralidharan A, Koenderink G H, Kreutzer M T, Boukany P E 2019 Sci. Rep. 9 8151 Google Scholar
[56]	Chafai D E, Sulimenko V, Havelka D, Kubínová L, Dráber P, Cifra M 2019 Adv. Mater. 31 1903636 Google Scholar
[57]	Chafai D E, Vostárek F, Dráberová E, Havelka D, Arnaud-Cormos D, Leveque P, Janáček J, Kubínová L, Cifra M, Dráber P 2020 Adv. Biosyst. 4 2000070 Google Scholar
[58]	Thompson G L, Roth C C, Kuipers M A, Tolstykh G P, Beier H T, Ibey B L 2016 Biochem. Biophys. Res. Commun. 470 35 Google Scholar
[59]	Xiao D, Tang L, Zeng C, Wang J, Luo X, Yao C, Sun C 2011 Cell Biol. Int. 35 99 Google Scholar
[60]	Ford W E, Ren W, Blackmore P F, Schoenbach K H, Beebe S J 2010 Arch. Biochem. Biophys. 497 82 Google Scholar
[61]	Steuer A, Wende K, Babica P, Kolb J F 2017 Eur. Biophys. J. Biophys. 46 567 Google Scholar
[62]	Rassokhin M A, Pakhomov A G 2012 J. Membr. Biol. 245 521 Google Scholar
[63]	Ren W, Sain N M, Beebe S J 2012 Biochem. Biophys. Res. Commun. 421 808 Google Scholar
[64]	Beebe S J 2015 Bioelectrochemistry 103 52 Google Scholar
[65]	Morotomi-Yano K, Yano K I, Research B 2015 Biochem. Biotechnol. Res. 3 51
[66]	Mao Z, Zhang Y, Lu N, Cheng S, Hong R, Liu Q H 2019 Small 16 1904047 Google Scholar
[67]	Nuccitelli R, Tran K, Sheikh S, Athos B, Kreis M, Nuccitelli P 2010 Int. J. Cancer 127 1727 Google Scholar
[68]	Beebe S J, Fox P M, Rec L J, Willis L K, Schoenbach K H 2003 FASEB J. 17 1 Google Scholar
[69]	Awasthi K, Nakabayashi T, Ohta N 2016 ACS Omega 1 396 Google Scholar
[70]	Taibi A, Perrin M L, Albouys J, Jacques J, Yardin C, Durand-Fontanier S, Bardet S M 2021 Clin. Transl. Oncol. 23 1220 Google Scholar
[71]	Nuccitelli R, McDaniel A, Anand S, Cha J, Mallon Z, Berridge J C, Uecker D 2017 J. Immunother Cancer 5 1 Google Scholar
[72]	Guo S, Jing Y, Burcus N I, Lassiter B P, Tanaz R, Heller R, Beebe S J 2018 Int. J. Cancer 142 629 Google Scholar
[73]	Vernier P T, Sun Y, Marcu L, Salemi S, Craft C M, Gundersen M A 2003 Biochem. Biophys. Res. Commun. 310 286 Google Scholar
[74]	Buescher E S, Smith R R, Schoenbach K H 2004 IEEE Trans. Plasma Sci. 32 1563 Google Scholar
[75]	Beebe S J, Blackmore P F, White J, Joshi R P, Schoenbach K H 2004 Physiol. Meas. 25 1077 Google Scholar
[76]	Zhang J, Blackmore P F, Hargrave B Y, Xiao S, Beebe S J, Schoenbach K H 2008 Arch. Biochem. Biophys. 471 240 Google Scholar
[77]	Wang S F, Chen J X, Chen M T, Vernier P T, Gundersen M A, Valderrabano M 2009 Biophys. J. 96 1640 Google Scholar
[78]	Beier H T, Roth C C, Tolstykh G P, Ibey B L 2012 Biochem. Biophys. Res. Commun. 423 863 Google Scholar
[79]	Semenov I, Xiao S, Pakhomov A G 2013 Biochim. Biophys. Acta Biomembr. 1828 981 Google Scholar
[80]	Pakhomova O N, Gregory B, Semenov I, Pakhomov A G 2014 Biochim. Biophys. Acta Biomembr. 1838 2547 Google Scholar
[81]	Fukuda H, Miyake M, Hirai H, Teranishi K, Shimomura N, Oyadomari S 2015 IEEE Pulsed Power Conference (PPC) Austin, Texas, USA, May 31-June 4, 2015 p1
[82]	Zhou P, He F, Han Y, Liu B, Wei S 2018 Bioelectrochemistry 124 7 Google Scholar
[83]	Furumoto Y, Sato D, Teranishi K, Shimomura N, Hamada Y, Miyake M, Oyadomari S 2018 IEEE International Power Modulator and High Voltage Conference (IPMHVC) Jackson, Wyoming, USA, June 3–7, 2018 p456
[84]	Zaklit J, Chat Te Rjee I, Leblanc N, Craviso G L 2019 Biochim. Biophys. Acta Biomembr. 1861 1180 Google Scholar
[85]	Ohnishi N, Fujiwara Y, Kamezaki T, Katsuki S 2019 IEEE Trans. Bio-Med. Eng. 66 2259 Google Scholar
[86]	Liu J, Lu Q, Liang R, Guo J, Wang K, Dong F, Wang J, Zhang J, Fang J 2019 Phys. Rev. Appl. 11 024001 Google Scholar
[87]	Jin D Y, Hamada Y, Furumoto Y, Izutani A, Taniuchi S, Miyake M, Oyadomari M, Teranishi K, Shimomura N, Oyadomari S 2020 Plos One 15 e0229948 Google Scholar
[88]	Semenov I, Casciola M, Ibey B L, Xiao S, Pakhomov A G 2018 Bioelectrochemistry 121 135 Google Scholar
[89]	Chen N, Schoenbach K H, Kolb J F, James Swanson R, Garner A L, Yang J, Joshi R P, Beebe S J 2004 Biochem. Biophys. Res. Commun. 317 421 Google Scholar
[90]	Nuccitelli R, Pliquett U, Chen X, Ford W, Swanson R, Beebe S, Kolb J, Schoenbach K 2006 Biochem. Biophys. Res. Commun. 343 351 Google Scholar
[91]	Nuccitelli R, Chen X, Pakhomov A G, Baldwin W H, Sheikh S, Pomicter J L, Ren W, Osgood C, Swanson R J, Kolb J F, Beebe S J, Schoenbach K H 2009 Int. J. Cancer 125 438 Google Scholar
[92]	Chen N, Garner A L, Chen G, Jing Y, Deng Y, Swanson R J, Kolb J F, Beebe S J, Joshi R P, Schoenbach K H 2007 Biochem. Biophys. Res. Commun. 364 220 Google Scholar
[93]	Ibey B L, Pakhomov A G, Gregory B W, Khorokhorina V A, Roth C C, Rassokhin M A, Bernhard J A, Wilmink G J, Pakhomova O N 2010 Biochim. Biophys. Acta General Subjects 1800 1210 Google Scholar
[94]	Ren W, Beebe S J 2011 Apoptosis 16 382 Google Scholar
[95]	Beebe J S 2015 J. Nanomed. Res. 2 00016 Google Scholar
[96]	Muratori C, Pakhomov A G, Gianulis E, Meads J, Casciola M, Mollica P A, Pakhomova O N 2017 J. Biol. Chem. 292 19381 Google Scholar
[97]	Chang A Y, Liu X, Tian H, Hua L, Yang Z, Wang S 2020 Sci. Rep. 10 1 Google Scholar
[98]	Stacey M, Stickley J, Fox P, Statler V, Schoenbach K, Beebe S J, Buescher S 2003 Mutat. Res-Gen. Tox. En. 542 65 Google Scholar
[99]	Beebe S J, White J, Blackmore P F, Deng Y, Somers K, Schoenbach K H 2003 DNA Cell Biol. 22 785 Google Scholar
[100]	Schoenbach K H, Be Ebe S J, Buescher E S 2010 Bioelectromagnetics 22 440 Google Scholar
[101]	Tekle E, Oubrahim H, Dzekunov S M, Kolb J F, Schoenbach K H, Chock P B 2005 Biophys. J. 89 274 Google Scholar
[102]	Humphrey J D, Dufresne E R, Schwartz M A 2014 Nat. Rev. Mol. Cell Biol. 15 802 Google Scholar
[103]	Paszek M J, Zahir N, Johnson K R, Lakins J N, Rozenberg G I, Gefen A, Reinhart-King C A, Margulies S S, Dembo M, Boettiger D, Hammer D A, Weaver V M 2005 Cancer Cell 8 241 Google Scholar
[104]	肖英, 程爱兰 2016 中国肿瘤临床 43 1007 Google Scholar Xiao Y, Cheng A L 2016 Chin. J. Clin. Oncol. 43 1007 Google Scholar
[105]	Haberkorn I, Siegenthaler L, Buchmann L, Neutsch L, Mathys A 2021 Biotechnol. Adv. 53 107780 Google Scholar
[106]	Havelka D, Chafai D E, Krivosudský O, Klebanovych A, Vostárek F, Kubínová L, Dráber P, Cifra M 2019 Adv. Mater. Technol. 5 1900669 Google Scholar
[107]	Chopinet L, Roduit C, Rols M P, Dague E 2013 Biochim. Biophys. ActaBiomembr. 1828 2223 Google Scholar
[108]	Louise C, Etienne D, Marie-Pierre R 2014 Cytoskeleton 71 587 Google Scholar
[109]	Svitkina T 2018 Csh. Perspect. Biol. 10 a018267 Google Scholar
[110]	Shi F, Steuer A, Zhuang J, Kolb J F 2019 IEEE Trans. Bio-Med. Eng. 66 2010 Google Scholar
[111]	Shi F, Zhuang J, Kolb J F 2019 J. Phys. D:Appl. Phys. 52 495401 Google Scholar
[112]	Shi F, Kolb J F 2020 Biosens. Bioelectron. 157 112149 Google Scholar
[113]	刘泰槰 1999 中国科学基金 1999 50 Google Scholar Liu T P 1999 Sci. Found. China 1999 50 Google Scholar
[114]	Lewis R S 2001 Annu. Rev. Immunol. 19 497 Google Scholar
[115]	Feske S 2007 Nat. Rev. Immunol. 7 690 Google Scholar
[116]	Nanosecond Pulsed Electric Fields Activate Intracellular Signaling Pathways. Tolstykh G P, Thompson T, Beier H T, Roth C C, Ibey B L https://spie.org/news/4736-nanosecond-pulsed-electric-fields-activate-intracellular-signaling-pathways?SSO=1 [2021-11-24]
[117]	陈娜子, 姜潮, 李校堃 2016 中国生物工程杂志 36 76 Google Scholar Chen N Z, Jiang C, Li X K 2016 China Biotechnology 36 76 Google Scholar
[118]	Liu J, Chen X, Zheng S 2021 Front. Med. 15 170 Google Scholar
[119]	Batista Napotnik T, Polajzer T, Miklavcic D 2021 Bioelectrochemistry 141 107871 Google Scholar
[120]	Zharkova L P, Romanchenko I V, Buldakov M A, Priputnev P V, Bolshakov M A, Rostov V V 2018 20th International Symposium on High-Current Electronics (ISHCE) Tomsk, Russia, September 16–22, 2018 p158
[121]	Nuccitelli R, McDaniel A, Connolly R, Zelickson B, Hartman H 2020 Laser Surg. Med. 52 882 Google Scholar
[122]	Tolstykh G P, Cantu J C, Tarango M, Ibey B L 2019 Biochim. Biophys. Acta Biomembr. 1861 685 Google Scholar
[123]	Awasthi K, Chang F L, Hsu H Y, Ohta N 2021 Sens. Actuators, B 347 130635 Google Scholar
[124]	Pakhomova O N, Khorokhorina V A, Bowman A M, Rodaitė-Riševičienė R, Saulis G, Xiao S, Pakhomov A G 2012 Arch. Biochem. Biophys. 527 55 Google Scholar
[125]	Guo S, Burcus N I, Scott M, Jing Y, Semenov I 2021 Sci. Rep. 11 23745
[126]	高薇, 侯微, 李伟, 王玉帅, 王英平 2014 中国畜牧兽医 41 150 Gao W, Hou W, Li W, Wang Y S, Wang Y P 2014 China Anim. Husb. Vet. Med. 41 150
[127]	Zhuang J, Steuer A, Jing Y, Kolb J F 2012 IEEE International Power Modulator and High Voltage Conference (IPMHVC) San Diego, California, USA, June 3–7, 2012 p106
[128]	岑超, 陈新华, 郑树森 2015 浙江大学学报(医学版) 44 678 Google Scholar Cen C, Chen X H, Zheng S S 2015 J. Zhejiang Univ., Med. Sci. 44 678 Google Scholar
[129]	Kielbik A, Szlasa W, Novickij V, Szewczyk A, Maciejewska M, Saczko J, Kulbacka J 2021 Sci. Rep. 11 15835 Google Scholar
[130]	Perrier D L, Rems L, Boukany P E 2017 Adv. Colloid Interface Sci. 249 248 Google Scholar
[131]	Qian J, Chen T, Wu Q, Zhou L, Zhou W, Wu L, Wang S, Lu J, Wang W, Li D, Xie H, Su R, Guo D, Liu Z, He N, Yin S, Zheng S 2021 Cancer Lett. 498 242 Google Scholar
[132]	Dan T, Okamo T, Abe K, Katsuki S, Sakugawa T, Akiyama H 2010 Abstracts IEEE International Conference on Plasma Science Norfolk, Virginia, USA, June 20–24, 2010 p1
[133]	Della Valle E, Marracino P, Pakhomova O, Liberti M, Apollonio F 2019 PloS One 14 e0221685 Google Scholar
[134]	Guo J S, Dong F H, Ding L, Wang K L, Zhang J, Fang J 2018 Bioelectrochemistry 123 26 Google Scholar

Cited By

图 1 PEFs导致的细胞膜EP示意图

Figure 1. Schematic diagram of PEF-induced membrane electroporation.

DownLoad: Full-Size Img PowerPoint

图 2 PEFs中哺乳动物细胞等效电路模型

Figure 2. Equivalent circuit model for the mammalian cells with the exposure to PEFs, where individual subcellular components are described by a combination of the resistor or capacitor.

DownLoad: Full-Size Img PowerPoint

图 3 NsPEFs细胞器效应的文献发表情况

Figure 3. Literature research about the intracellular effects for cells exposured to nsPEFs.

DownLoad: Full-Size Img PowerPoint

图 4 NsPEFs对贴壁细胞的影响. 1, 破坏细胞与细胞间连接; 2, 细胞膜电穿孔; 3, 细胞脱落

Figure 4. Effect of nsPEFs on adherent cells. 1, Disruption of the intercallular connections; 2, electroporation; 3, cell necrosis or shedding.

DownLoad: Full-Size Img PowerPoint

图 5 NsPEFs诱导的细胞内凋亡途径

Figure 5. Apoptosis pathways for cells after exposure to nsPEFs

DownLoad: Full-Size Img PowerPoint

表 1 nsPEFs的细胞器生物电效应总结

Table 1. Summary of effects of nsPEFs on cell organelles.

细胞器	细胞类型	脉冲宽度/ns	电场强度/(kV·cm^–1)	主要结果概述
细胞骨架	GH3, HeLa^[47]	60	12	肌动蛋白丝变短、变细、碎片化、解聚、收缩、分离, 细胞弹性降低; 微管屈曲、解聚、破碎; 微管聚合速率和聚合数量发生变化; 中间丝破坏; 细胞通透性改变; 细胞肿胀、起泡、细胞质颗粒化; 细胞间通讯受抑制; 细胞骨架的破坏受钙离子调控: 1)高钙离子浓度溶液中, nsPEFs处理会使微管解聚, 破坏肌动蛋白丝; 2) 低钙离子浓度溶液中, nsPEFs处理后微管显示正常结构
	BY-2^[48]	10	33
	Jurkat, HeLa, SV40^[49]	60	15, 60
	CHO-K1^[50]	600	19.2
	Jurkat, U937, CHO-K1^[51]	10	150
	WB-F344^[52]	100	5—35
	U87-MG^[53]	10, 100	44
	CHO^[54]	600	16.2
	GUV^[55]		3—10
	tubulin^[56]	10	20
	U2OS^[57]
	CHO-K1^[58]	10, 60	27.7, 150
	HepG2^[59]	450	8
	B16-F10^[60]	300	12, 18, 26, 40, 60
	WB-F344, WB-Ras^[61]	100	20
	U-937^[62]	60	10
线粒体	Jurkat^[63]	60	0—60	线粒体膜通透性改变, 线粒体的通透性转换孔(MPTP)不可逆过度开放; 线粒体膜电位损失; 线粒体肿胀; 线粒体膜蛋白受影响, 线粒体膜间隙蛋白 Cyt-C, AIF 释放入胞浆; 调控线粒体凋亡途径, Caspase-3表达量增加, Bax 表达量增加; 线粒体释放细胞色素C; 影响线粒体信号传导途径; ATP消耗; 胞内ROS水平升高
	N1-S1^[36]	600	0—80
	Jurkat, U-937^[46]	10	50, 150
	Jurkat^[64]	600	0—60
	HeLa S3^[65]	80	20
	HCT116, NCM460^[66]	10, 600, 800	3, 4, 5
	Jurkat, B10-2^[67]	10—300	≤ 300
	Jurkat, HL-60^[68]	10, 60, 300	150, 60, 25
	Jurkat, HL-60^[34]	10—300	15—60
	Hela^[69]	10, 20, 30, 50	40, 45
	CT-26 tumor cells^[70]	10	22
	MCA205, McA-RH7777, JurkatE6-1^[71]	100	6—25
	4T1^[72]	100	46—54
内质网	Jurkat^[73]	7, 10, 30	25	内质网穿孔、损伤; 钙离子释放, 引发胞内钙离子浓度升高; 内质网应激响应; 免疫原性细胞死亡; 肿瘤细胞内与内质网凋亡相关蛋白Caspase-3的释放量增加; 内质网凋亡信号通路起作用
	Jurkat, HL-60^[74]	60, 300	15—60
	Jurkat, HL-60^[75]	10, 60, 300	26, 40, 60, 150, 300 (10 ns); 16, 26, 40, 60 (60 ns); 40 (300 ns)
	Newly outdated platelet^[76]	300	0—30
	Cardiac cells from rats^[77]	4	10—80
	Jurkat^[15]	60	25, 50, 100
	NG108-15^[78]	4	16.2
	CHO-K1^[79]	60	3.7—30
	U937, CHO-K1, BPAE^[80]	300
	HeLa, HEK293T, C2C12^[40]	7, 10, 20	10—50
	HeLa, HEK293, MEF^[81]	14	10, 20, 25, 30, 40, 50
	MG63^[82]	60	6.7, 13.3, 16.7, 20, 26.7, 33.3
	HeLa, MEF^[83]	14, 70	80, 100 (14 ns), 30, 50, 70, 75 (70 ns)
	Bovine chromaffin cells ^[84]	5	170
	B16 F10, EL-4^[39]	200	7
	Hela^[85]	20, 500	100, 20
	Murine secondary oocytes ^[86]	10	4—10
	MEF^[87]	60—300	30, 60
	CHO-K1, NG108^[88]	300, 600	3.7, 7.4, 11, 1
细胞核	HL-60^[89]	10, 60	65 (10 ns), 25 (60 ns)	核膜穿孔; DNA双链破坏、DNA片段化; 选择性降低DNA甲基化; 核蛋白复合物改变, 抑制snRNA的生成, 改变亚核结构
	B16 F10^[18,90,91]	300	40
	Jurkat^[92]	10	150
	Jurkat, U-937^[93]	10, 300	2.25, 4.5, 150, 290
	B16F10^[60]	300	0—60
	E4 squamous cell ^[94]	300	0—60
	Jurkat^[16]	60	10, 15, 25
	N1-S1^[17]	600	0—80
	N1-S1^[95]	100	50
	CHO^[58]	10, 600	18.2, 27.7, 16.7
	HEK 293^[96]	300	25.5
	HL-60^[36]	80	20
	K562, CT26. WT^[97]	600	50
	HL60, Jurkat, ALL^[98]	10, 60, 300	26, 60, 150, 300
	B10-2, HL-60^[99]	10, 50, 60, 300	26, 60, 75, 150
溶酶体	CHO-K1^[41]	1, 20, 600	16.2	溶酶体去膜化、溶酶体损伤; 溶酶体运动受影响, 高钙离子浓度溶液下, 溶酶体迁移停止;
溶酶体	CHO-K1^[42]	600	16.2	溶酶体去膜化、溶酶体损伤; 溶酶体运动受影响, 高钙离子浓度溶液下, 溶酶体迁移停止;
囊泡	Human eosinophils ^[100]	60	36, 53	囊泡穿孔; 诱导细胞外囊泡的释放
囊泡	COS-7^[101]	50	20—300	囊泡穿孔; 诱导细胞外囊泡的释放

DownLoad: CSV

[1]	高宇, 门业堃, 张建, 刘范嘉, 邓永迪, 王振 2016 高电压技术 42 2675 Google Scholar Gao Y, Men Y K, Zhang J, Liu F J, Deng Y D, Wang Z 2016 High Voltage Eng. 42 2675 Google Scholar
[2]	Amr A G, Schoenbch K H 2000 IEEE Trans. Plasma Sci. 28 115 Google Scholar
[3]	Sitzmann W, Vorobiev E, Lebovka N 2016 Innovative Food Sci. Emerg. 37 302 Google Scholar
[4]	Barbosa-Cánovas G V, Altunakar B 2006 Pulsed Electric Fields Technology for the Food Industry: Fundamentals and Applications (Boston: Springer US) pp3–26
[5]	Takaki K, Yoshida K, Saito T, Kusaka T, Yamaguchi R, Takahashi K, Sakamoto Y 2014 Microorganisms 2 58 Google Scholar
[6]	Takaki K, Yamazaki N, Mukaigawa S, Fujiwara T, Kofujita H, Takahasi K, Narimatsu M, Nagane K 2009 Acta Phys. Pol. A 115 1062 Google Scholar
[7]	Deore A B, Sapakal V D, Shikalgar T S, Jagtap M J, Bhinde C J 2017 Innovative Pharm. 5 147
[8]	Heller R, Jaroszeski M J, Reintgen D S, Puleo C A, DeConti R C, Gilbert R A, Glass L F 1998 Cancers 83 148 Google Scholar
[9]	Heller R, Gilbert R, Jaroszeski M J 1999 Adv. Drug Deliver. Rev. 35 119 Google Scholar
[10]	姚陈果, 宁郡怡, 刘红梅, 郑爽, 董守龙 2020 电工技术学报 35 115 Google Scholar Yao C G, Ning J Y, Liu H M, Zheng S, Dong S L 2020 Trans. China Electrotech. Soc. 35 115 Google Scholar
[11]	Golberg A, Broelsch G, Bohr S, Mihm M, Austen W, Albadawi H, Watkins M, Yarmush M 2013 Technology 1 1 Google Scholar
[12]	Golberg A, Yarmush M 2013 IEEE Trans. Bio-Med. Eng. 60 707 Google Scholar
[13]	Schoenbach K H, Joshi R P, Kolb J F, Nianyong C, Stacey M, Blackmore P F, Buescher E S, Beebe S J 2004 Proc. IEEE 92 1122 Google Scholar
[14]	Graybill P M, Davalos R V 2020 Cancers 12 1132 Google Scholar
[15]	Scarlett S S, White J A, Blackmore P F, Schoenbach K H, Kolb J F 2009 Biochim. Biophys. Acta Biomembr. 1788 1168 Google Scholar
[16]	Rubinsky B, Romeo S, Zeni L, Sarti M, Sannino A, Scarfì M R, Vernier P T, Zeni O 2011 PLoS One 6 e28419 Google Scholar
[17]	Beebe S, Sain N, Ren W 2013 Cells 2 136 Google Scholar
[18]	Chen X, Kolb J F, Swanson R J, Schoenbach K H, Beebe S J 2010 Pigm. Cell Melanoma R. 23 554 Google Scholar
[19]	Vadlamani R A, Nie Y, Detwiler D A, Dhanabal A, Kraft A M, Kuang S, Gavin T P, Garner A L 2019 J. R. Soc. Interface 16 20190079 Google Scholar
[20]	Chen J, Huang Y, Yang J, Li K, Jiang Y, Heng B C, Cai Q, Zhang J, Ge Z 2020 J. Tissue Eng. Regen. Med. 14 1136 Google Scholar
[21]	Kim V, Gudvangen E, Kondratiev O, Redondo L M, Xiao S, Pakhomov A 2021 Int. J. Mol. Sci. 22 7051 Google Scholar
[22]	Casciola M, Xiao S, Pakhomov A 2017 Sci. Rep. 7 10453
[23]	Tolstykh G P, Valdez C M, Montgomery N D, Cantu J C, Sedelnikova A, Ibey B L 2021 Bioelectrochemistry 142 107930 Google Scholar
[24]	Stampfli R 1958 An Acad. Brasil Ciens. 30 57
[25]	Neumann E, Schaeferridder M, Wang Y, Hofschneider P H 1982 EMBO. J. 1 841 Google Scholar
[26]	Weaver J C 1993 J. Cell. Biochem. 51 426 Google Scholar
[27]	Glaser R W, Leikin S L, Chernomordik L V, Pastushenko V F, Sokirko A I 1988 Biochim. Biophys. Acta Biomembr. 940 275 Google Scholar
[28]	Weaver J C, Chizmadzhev Y A 1996 Bioelectrochemistry 41 135
[29]	Somiari S, Glasspool-Malone J, Drabick J J, Gilbert R A, Heller R, Jaroszeski M J, Malone R W 2000 Mol. Ther. 2 178 Google Scholar
[30]	史秋生, 杜联芳, 张焱锋 2018 肿瘤影像学 27 242 Shi Q S, Du L F, Zhang Y F 2018 Oncoradiology 27 242
[31]	李小曼, 万利杰, 王晓娟, 谢红梅, 兰建, 刘晓云 2017 现代生物医学进展 17 1190 Google Scholar Li X M, Wan L J, Wang X J, Xie H M, Lan J, Liu X Y 2017 Prog. Mod. Biomed. 17 1190 Google Scholar
[32]	Deipolyi A R, Golberg A, Yarmush M L, Arellano R S, Oklu R 2014 Diagn. Interv. Radiol. 20 147 Google Scholar
[33]	庞小峰 2008 生物电磁学 (北京: 国防工业出版社) 第101页 Pang X F 2008 Bioelectromagnetism (Beijing: National Defense Industry Press) p101 (in Chinese)
[34]	Buescher E S, Schoenbach K H 2003 IEEE Trans. Dielectr. Electr. Insul. 10 788 Google Scholar
[35]	Koga T, Morotomi-Yano K, Sakugawa T, Saitoh H, Yano K I 2019 Sci. Rep. 9 8451 Google Scholar
[36]	Guerrero-Hernandez A, Beebe S J, Chen Y J, Sain N M, Schoenbach K H, Xiao S 2012 PLoS One 7 e51349 Google Scholar
[37]	Napotnik T B, Wu Y H, Gundersen M A, Miklavčič D, Vernier P T 2012 Bioelectromagnetics 33 257 Google Scholar
[38]	Jansen E D, Thomas R J, Wilmink G J, Ibey B L, Estlack L E, Roth C C, Cerna C Z, Wilmink G J, Ibey B L 2014 Proceedings of the SPIE San Francisco, California, USA, March 13, 2014 p89411S
[39]	Rossi A, Pakhomova O N, Mollica P A, Casciola M, Mangalanathan U, Pakhomov A G, Muratori C 2019 Cancers 11 2034 Google Scholar
[40]	Hirai H, Miyake M, Nagano A, Teranishi K, Shimomura N, Oyadomari S 2014 IEEE International Power Modulator and High Voltage Conference (IPMHVC) Santa Fe, New Mexico, USA, June 1–5, 2014 p419
[41]	Thompson G L, Roth C C, Dalzell D R, Kuipers M A, Ibey B L 2014 J. Biomed. Opt. 19 1 Google Scholar
[42]	Thompson G, Beier H, Ibey B 2018 Bioengineering 5 103 Google Scholar
[43]	Schoenbach K H, Katsuki S, Stark R H, Buescher E S, Beebe S J 2002 IEEE Trans. Plasma Sci. 30 293 Google Scholar
[44]	Schoenbach K H 2018 Bioelectromagnetics 39 257 Google Scholar
[45]	姚陈果, 莫登斌, 孙才新, 陈新, 熊正爱 2008 生物医学工程学杂志 25 1206 Google Scholar Yao C G, Mo D B, Sun C X, Chen X, Xiong Z A 2008 J. Biomed. Eng. 25 1206 Google Scholar
[46]	米彦 2009 博士学位论文 (重庆: 重庆大学) Mi Y 2009 Ph. D. Dissertation (Chongqing: Chongqing University) (in Chinese)
[47]	Pakhomov A G, Shevin R, White J A, Kolb J F, Pakhomova O N, Joshi R P, Schoenbach K H 2007 Arch. Biochem. Biophys. 465 109 Google Scholar
[48]	Berghöfer T, Eing C, Flickinger B, Hohenberger P, Wegner L H, Frey W, Nick P 2009 Biochem. Biophys. Res. Commun. 387 590 Google Scholar
[49]	Stacey M, Fox P, Buescher S, Kolb J 2011 Bioelectrochemistry 82 131 Google Scholar
[50]	Pakhomov A G, Xiao S, Pakhomova O N, Semenov I, Kuipers M A, Ibey B L 2014 Bioelectrochemistry 100 88 Google Scholar
[51]	Wilmink G J, Thompson G L, Roth C, Tolstykh G, Kuipers M, Ibey B L, Ibey B L 2013 Proceedings of the SPIE San Francisco, California, USA, February 23, 2013 p85850T
[52]	Steuer A, Schmidt A, Labohá P, Babica P, Kolb J F 2016 Bioelectrochemistry 112 33 Google Scholar
[53]	Carr L, Bardet S M, Burke R C, Arnaud-Cormos D, Leveque P, O'Connor R P 2017 Sci. Rep. 7 41267
[54]	Tolstykh G P, Thompson G L, Beier H T, Steelman Z A, Ibey B L 2017 BB Reports 9 36 Google Scholar
[55]	Perrier D L, Vahid A, Kathavi V, Stam L, Rems L, Mulla Y, Muralidharan A, Koenderink G H, Kreutzer M T, Boukany P E 2019 Sci. Rep. 9 8151 Google Scholar
[56]	Chafai D E, Sulimenko V, Havelka D, Kubínová L, Dráber P, Cifra M 2019 Adv. Mater. 31 1903636 Google Scholar
[57]	Chafai D E, Vostárek F, Dráberová E, Havelka D, Arnaud-Cormos D, Leveque P, Janáček J, Kubínová L, Cifra M, Dráber P 2020 Adv. Biosyst. 4 2000070 Google Scholar
[58]	Thompson G L, Roth C C, Kuipers M A, Tolstykh G P, Beier H T, Ibey B L 2016 Biochem. Biophys. Res. Commun. 470 35 Google Scholar
[59]	Xiao D, Tang L, Zeng C, Wang J, Luo X, Yao C, Sun C 2011 Cell Biol. Int. 35 99 Google Scholar
[60]	Ford W E, Ren W, Blackmore P F, Schoenbach K H, Beebe S J 2010 Arch. Biochem. Biophys. 497 82 Google Scholar
[61]	Steuer A, Wende K, Babica P, Kolb J F 2017 Eur. Biophys. J. Biophys. 46 567 Google Scholar
[62]	Rassokhin M A, Pakhomov A G 2012 J. Membr. Biol. 245 521 Google Scholar
[63]	Ren W, Sain N M, Beebe S J 2012 Biochem. Biophys. Res. Commun. 421 808 Google Scholar
[64]	Beebe S J 2015 Bioelectrochemistry 103 52 Google Scholar
[65]	Morotomi-Yano K, Yano K I, Research B 2015 Biochem. Biotechnol. Res. 3 51
[66]	Mao Z, Zhang Y, Lu N, Cheng S, Hong R, Liu Q H 2019 Small 16 1904047 Google Scholar
[67]	Nuccitelli R, Tran K, Sheikh S, Athos B, Kreis M, Nuccitelli P 2010 Int. J. Cancer 127 1727 Google Scholar
[68]	Beebe S J, Fox P M, Rec L J, Willis L K, Schoenbach K H 2003 FASEB J. 17 1 Google Scholar
[69]	Awasthi K, Nakabayashi T, Ohta N 2016 ACS Omega 1 396 Google Scholar
[70]	Taibi A, Perrin M L, Albouys J, Jacques J, Yardin C, Durand-Fontanier S, Bardet S M 2021 Clin. Transl. Oncol. 23 1220 Google Scholar
[71]	Nuccitelli R, McDaniel A, Anand S, Cha J, Mallon Z, Berridge J C, Uecker D 2017 J. Immunother Cancer 5 1 Google Scholar
[72]	Guo S, Jing Y, Burcus N I, Lassiter B P, Tanaz R, Heller R, Beebe S J 2018 Int. J. Cancer 142 629 Google Scholar
[73]	Vernier P T, Sun Y, Marcu L, Salemi S, Craft C M, Gundersen M A 2003 Biochem. Biophys. Res. Commun. 310 286 Google Scholar
[74]	Buescher E S, Smith R R, Schoenbach K H 2004 IEEE Trans. Plasma Sci. 32 1563 Google Scholar
[75]	Beebe S J, Blackmore P F, White J, Joshi R P, Schoenbach K H 2004 Physiol. Meas. 25 1077 Google Scholar
[76]	Zhang J, Blackmore P F, Hargrave B Y, Xiao S, Beebe S J, Schoenbach K H 2008 Arch. Biochem. Biophys. 471 240 Google Scholar
[77]	Wang S F, Chen J X, Chen M T, Vernier P T, Gundersen M A, Valderrabano M 2009 Biophys. J. 96 1640 Google Scholar
[78]	Beier H T, Roth C C, Tolstykh G P, Ibey B L 2012 Biochem. Biophys. Res. Commun. 423 863 Google Scholar
[79]	Semenov I, Xiao S, Pakhomov A G 2013 Biochim. Biophys. Acta Biomembr. 1828 981 Google Scholar
[80]	Pakhomova O N, Gregory B, Semenov I, Pakhomov A G 2014 Biochim. Biophys. Acta Biomembr. 1838 2547 Google Scholar
[81]	Fukuda H, Miyake M, Hirai H, Teranishi K, Shimomura N, Oyadomari S 2015 IEEE Pulsed Power Conference (PPC) Austin, Texas, USA, May 31-June 4, 2015 p1
[82]	Zhou P, He F, Han Y, Liu B, Wei S 2018 Bioelectrochemistry 124 7 Google Scholar
[83]	Furumoto Y, Sato D, Teranishi K, Shimomura N, Hamada Y, Miyake M, Oyadomari S 2018 IEEE International Power Modulator and High Voltage Conference (IPMHVC) Jackson, Wyoming, USA, June 3–7, 2018 p456
[84]	Zaklit J, Chat Te Rjee I, Leblanc N, Craviso G L 2019 Biochim. Biophys. Acta Biomembr. 1861 1180 Google Scholar
[85]	Ohnishi N, Fujiwara Y, Kamezaki T, Katsuki S 2019 IEEE Trans. Bio-Med. Eng. 66 2259 Google Scholar
[86]	Liu J, Lu Q, Liang R, Guo J, Wang K, Dong F, Wang J, Zhang J, Fang J 2019 Phys. Rev. Appl. 11 024001 Google Scholar
[87]	Jin D Y, Hamada Y, Furumoto Y, Izutani A, Taniuchi S, Miyake M, Oyadomari M, Teranishi K, Shimomura N, Oyadomari S 2020 Plos One 15 e0229948 Google Scholar
[88]	Semenov I, Casciola M, Ibey B L, Xiao S, Pakhomov A G 2018 Bioelectrochemistry 121 135 Google Scholar
[89]	Chen N, Schoenbach K H, Kolb J F, James Swanson R, Garner A L, Yang J, Joshi R P, Beebe S J 2004 Biochem. Biophys. Res. Commun. 317 421 Google Scholar
[90]	Nuccitelli R, Pliquett U, Chen X, Ford W, Swanson R, Beebe S, Kolb J, Schoenbach K 2006 Biochem. Biophys. Res. Commun. 343 351 Google Scholar
[91]	Nuccitelli R, Chen X, Pakhomov A G, Baldwin W H, Sheikh S, Pomicter J L, Ren W, Osgood C, Swanson R J, Kolb J F, Beebe S J, Schoenbach K H 2009 Int. J. Cancer 125 438 Google Scholar
[92]	Chen N, Garner A L, Chen G, Jing Y, Deng Y, Swanson R J, Kolb J F, Beebe S J, Joshi R P, Schoenbach K H 2007 Biochem. Biophys. Res. Commun. 364 220 Google Scholar
[93]	Ibey B L, Pakhomov A G, Gregory B W, Khorokhorina V A, Roth C C, Rassokhin M A, Bernhard J A, Wilmink G J, Pakhomova O N 2010 Biochim. Biophys. Acta General Subjects 1800 1210 Google Scholar
[94]	Ren W, Beebe S J 2011 Apoptosis 16 382 Google Scholar
[95]	Beebe J S 2015 J. Nanomed. Res. 2 00016 Google Scholar
[96]	Muratori C, Pakhomov A G, Gianulis E, Meads J, Casciola M, Mollica P A, Pakhomova O N 2017 J. Biol. Chem. 292 19381 Google Scholar
[97]	Chang A Y, Liu X, Tian H, Hua L, Yang Z, Wang S 2020 Sci. Rep. 10 1 Google Scholar
[98]	Stacey M, Stickley J, Fox P, Statler V, Schoenbach K, Beebe S J, Buescher S 2003 Mutat. Res-Gen. Tox. En. 542 65 Google Scholar
[99]	Beebe S J, White J, Blackmore P F, Deng Y, Somers K, Schoenbach K H 2003 DNA Cell Biol. 22 785 Google Scholar
[100]	Schoenbach K H, Be Ebe S J, Buescher E S 2010 Bioelectromagnetics 22 440 Google Scholar
[101]	Tekle E, Oubrahim H, Dzekunov S M, Kolb J F, Schoenbach K H, Chock P B 2005 Biophys. J. 89 274 Google Scholar
[102]	Humphrey J D, Dufresne E R, Schwartz M A 2014 Nat. Rev. Mol. Cell Biol. 15 802 Google Scholar
[103]	Paszek M J, Zahir N, Johnson K R, Lakins J N, Rozenberg G I, Gefen A, Reinhart-King C A, Margulies S S, Dembo M, Boettiger D, Hammer D A, Weaver V M 2005 Cancer Cell 8 241 Google Scholar
[104]	肖英, 程爱兰 2016 中国肿瘤临床 43 1007 Google Scholar Xiao Y, Cheng A L 2016 Chin. J. Clin. Oncol. 43 1007 Google Scholar
[105]	Haberkorn I, Siegenthaler L, Buchmann L, Neutsch L, Mathys A 2021 Biotechnol. Adv. 53 107780 Google Scholar
[106]	Havelka D, Chafai D E, Krivosudský O, Klebanovych A, Vostárek F, Kubínová L, Dráber P, Cifra M 2019 Adv. Mater. Technol. 5 1900669 Google Scholar
[107]	Chopinet L, Roduit C, Rols M P, Dague E 2013 Biochim. Biophys. ActaBiomembr. 1828 2223 Google Scholar
[108]	Louise C, Etienne D, Marie-Pierre R 2014 Cytoskeleton 71 587 Google Scholar
[109]	Svitkina T 2018 Csh. Perspect. Biol. 10 a018267 Google Scholar
[110]	Shi F, Steuer A, Zhuang J, Kolb J F 2019 IEEE Trans. Bio-Med. Eng. 66 2010 Google Scholar
[111]	Shi F, Zhuang J, Kolb J F 2019 J. Phys. D:Appl. Phys. 52 495401 Google Scholar
[112]	Shi F, Kolb J F 2020 Biosens. Bioelectron. 157 112149 Google Scholar
[113]	刘泰槰 1999 中国科学基金 1999 50 Google Scholar Liu T P 1999 Sci. Found. China 1999 50 Google Scholar
[114]	Lewis R S 2001 Annu. Rev. Immunol. 19 497 Google Scholar
[115]	Feske S 2007 Nat. Rev. Immunol. 7 690 Google Scholar
[116]	Nanosecond Pulsed Electric Fields Activate Intracellular Signaling Pathways. Tolstykh G P, Thompson T, Beier H T, Roth C C, Ibey B L https://spie.org/news/4736-nanosecond-pulsed-electric-fields-activate-intracellular-signaling-pathways?SSO=1 [2021-11-24]
[117]	陈娜子, 姜潮, 李校堃 2016 中国生物工程杂志 36 76 Google Scholar Chen N Z, Jiang C, Li X K 2016 China Biotechnology 36 76 Google Scholar
[118]	Liu J, Chen X, Zheng S 2021 Front. Med. 15 170 Google Scholar
[119]	Batista Napotnik T, Polajzer T, Miklavcic D 2021 Bioelectrochemistry 141 107871 Google Scholar
[120]	Zharkova L P, Romanchenko I V, Buldakov M A, Priputnev P V, Bolshakov M A, Rostov V V 2018 20th International Symposium on High-Current Electronics (ISHCE) Tomsk, Russia, September 16–22, 2018 p158
[121]	Nuccitelli R, McDaniel A, Connolly R, Zelickson B, Hartman H 2020 Laser Surg. Med. 52 882 Google Scholar
[122]	Tolstykh G P, Cantu J C, Tarango M, Ibey B L 2019 Biochim. Biophys. Acta Biomembr. 1861 685 Google Scholar
[123]	Awasthi K, Chang F L, Hsu H Y, Ohta N 2021 Sens. Actuators, B 347 130635 Google Scholar
[124]	Pakhomova O N, Khorokhorina V A, Bowman A M, Rodaitė-Riševičienė R, Saulis G, Xiao S, Pakhomov A G 2012 Arch. Biochem. Biophys. 527 55 Google Scholar
[125]	Guo S, Burcus N I, Scott M, Jing Y, Semenov I 2021 Sci. Rep. 11 23745
[126]	高薇, 侯微, 李伟, 王玉帅, 王英平 2014 中国畜牧兽医 41 150 Gao W, Hou W, Li W, Wang Y S, Wang Y P 2014 China Anim. Husb. Vet. Med. 41 150
[127]	Zhuang J, Steuer A, Jing Y, Kolb J F 2012 IEEE International Power Modulator and High Voltage Conference (IPMHVC) San Diego, California, USA, June 3–7, 2012 p106
[128]	岑超, 陈新华, 郑树森 2015 浙江大学学报(医学版) 44 678 Google Scholar Cen C, Chen X H, Zheng S S 2015 J. Zhejiang Univ., Med. Sci. 44 678 Google Scholar
[129]	Kielbik A, Szlasa W, Novickij V, Szewczyk A, Maciejewska M, Saczko J, Kulbacka J 2021 Sci. Rep. 11 15835 Google Scholar
[130]	Perrier D L, Rems L, Boukany P E 2017 Adv. Colloid Interface Sci. 249 248 Google Scholar
[131]	Qian J, Chen T, Wu Q, Zhou L, Zhou W, Wu L, Wang S, Lu J, Wang W, Li D, Xie H, Su R, Guo D, Liu Z, He N, Yin S, Zheng S 2021 Cancer Lett. 498 242 Google Scholar
[132]	Dan T, Okamo T, Abe K, Katsuki S, Sakugawa T, Akiyama H 2010 Abstracts IEEE International Conference on Plasma Science Norfolk, Virginia, USA, June 20–24, 2010 p1
[133]	Della Valle E, Marracino P, Pakhomova O, Liberti M, Apollonio F 2019 PloS One 14 e0221685 Google Scholar
[134]	Guo J S, Dong F H, Ding L, Wang K L, Zhang J, Fang J 2018 Bioelectrochemistry 123 26 Google Scholar

[1]	Wang Lei, Ma Xi-Yue, Chen Ke-An, Liu Tao. Low frequency sound absorption performance of large sized active micro-perforated panel absorber in free field. Acta Physica Sinica, 2023, 72(6): 064304. doi: 10.7498/aps.72.20222151
[2]	Zhuang Jie, Han Rui, Ji Zhen-Yu, Shi Fu-Kun. Uncertainty in prediction of pulsed field ablation caused by parameter diversity in quantifying conductivity models. Acta Physica Sinica, 2023, 72(14): 147701. doi: 10.7498/aps.72.20230203
[3]	. Research progress on biological effects of cell membrane under infrared and terahertz irradiation. Acta Physica Sinica, 2022, (): . doi: 10.7498/aps.71.20212030
[4]	Wang Cheng-Yao, Li Xu, Lu Xiao-Yun. Fabrication of cyclic olefin polymer and polydimethylsiloxane co-bonded microfluidic device and its appliactions in terahertz biological effects on intestinal cells. Acta Physica Sinica, 2021, 70(24): 248706. doi: 10.7498/aps.70.20211807
[5]	Bo Wen-Fei, Che Rong, Kong Lei, Zhang Ming-Jie, Zhang Xiao-Bo. Research progress of biological effects of cell membrane under infrared and terahertz irradiation. Acta Physica Sinica, 2021, 70(24): 248707. doi: 10.7498/aps.70.20212030
[6]	Li Yuan, Li Lin-Bo, Wen Jia-Ye, Ni Zheng-Quan, Zhang Guan-Jun. Initiation of nanosecond-pulsed discharge in water: Electrostriction effect. Acta Physica Sinica, 2021, 70(2): 024701. doi: 10.7498/aps.70.20201048
[7]	Yao Jia-Feng, Wan Jian-Fen, Yang Lu, Liu Kai, Chen Bai, Wu Hong-Tao. Electrical characteristics of cells with electrical impedance spectroscopy. Acta Physica Sinica, 2020, 69(16): 163301. doi: 10.7498/aps.69.20200601
[8]	Tian Xiao-Fei, Zhang Xin. Biological effects on cells in strong static magnetic field. Acta Physica Sinica, 2018, 67(14): 148701. doi: 10.7498/aps.67.20180378
[9]	Wang Xiao-Fa, Zhang Jun-Hong, Gao Zi-Ye, Xia Guang-Qiong, Wu Zheng-Mao. Nanosecond mode-locked Tm-doped fiber laser based on graphene saturable absorber. Acta Physica Sinica, 2017, 66(11): 114209. doi: 10.7498/aps.66.114209
[10]	Qiu Ji-Si, Tang Xiong-Xin, Fan Zhong-Wei, Chen Yan-Zhong, Ge Wen-Qi, Wang Hao-Cheng, Liu Hao. High repetition rate and high beam quality joule level Nd: YAG nanosecond laser for Thomson scattering diagnosis. Acta Physica Sinica, 2016, 65(15): 154204. doi: 10.7498/aps.65.154204
[11]	Sheng Liang, Li Yang, Wu Jian, Yuan Yuan, Zhao Ji-Zhen, Zhang Mei, Peng Bo-Dong, Hei Dong-Wei. Nanosecond electrical explosion of twisted aluminum wires. Acta Physica Sinica, 2014, 63(20): 205203. doi: 10.7498/aps.63.205203
[12]	Li Yuan, Mu Hai-Bao, Deng Jun-Bo, Zhang Guan-Jun, Wang Shu-Hong. Simulational study on streamer discharge in transformer oil under positive nanosecond pulse voltage. Acta Physica Sinica, 2013, 62(12): 124703. doi: 10.7498/aps.62.124703
[13]	Yang Hong-Dao, Li Xiao-Hong, Li Guo-Qiang, Yuan Chun-Hua, Tang Duo-Chang, Xu Qin, Qiu Rong, Wang Jun-Bo. Silicon surface microstructures created by 1064 nm Nd∶YAG nanosecond laser. Acta Physica Sinica, 2011, 60(2): 027901. doi: 10.7498/aps.60.027901
[14]	Lü Xiao-Gui, Ren Chun-Sheng, Ma Teng-Cai, Zhu Hai-Long, Qian Mu-Yang, Wang De-Zhen. Influence of quartz tube on the nanosecond pulsed discharge in a cone-to-plane air gap. Acta Physica Sinica, 2010, 59(11): 7917-7921. doi: 10.7498/aps.59.7917
[15]	Song You-Jian, Hu Ming-Lie, Xie Chen, Chai Lu, Wang Qing-Yue. Approaching 100 nJ pulse energy output from a mode-locked photonic crystal fiber laser. Acta Physica Sinica, 2010, 59(10): 7105-7110. doi: 10.7498/aps.59.7105
[16]	Han Jing-Hua, Feng Guo-Ying, Yang Li-Ming, Zhang Qiu-Hui, Jia Jun, Li Gang, Zhu Qi-Hua, Zhou Shou-Huan. Study on the critical free electron density for nanosecond laser pulse focusing in air. Acta Physica Sinica, 2008, 57(10): 6304-6310. doi: 10.7498/aps.57.6304
[17]	Hu Jing-Dan, Cai Jing, Chen Jun-Rong, Li Quan, Zhao Ke-Qing. Study on charge transport properties of hexaazatriphenylene and its derivatives. Acta Physica Sinica, 2008, 57(9): 5464-5468. doi: 10.7498/aps.57.5464
[18]	Shao Tao, Sun Guang-Sheng, Yan Ping, Gu Chen, Zhang Shi-Chang. Calculation on runaway process of high-energy fast electrons under nanosecond-pulse. Acta Physica Sinica, 2006, 55(11): 5964-5968. doi: 10.7498/aps.55.5964
[19]	Tan Xin-Yu, Zhang Duan-Ming, Li Zhi-Hua, Guan Li, Li Li. Target ablation characteristics of thin films during nanosecond pulsed laser deposition in the ablation process. Acta Physica Sinica, 2005, 54(8): 3915-3921. doi: 10.7498/aps.54.3915
[20]	Sun Da-Rui, Song Yan-Rong, Zhang Zhi-Gang, Liu Yong-Jun, Chai Lu, Wang Qing-Yue. Compare of characteristics between Martinez and Offner stretchers used in chirped pulse amplifier. Acta Physica Sinica, 2003, 52(4): 870-874. doi: 10.7498/aps.52.870

Catalog

Vol. 71, Issue 6 - 2022-03-20

Metrics

Abstract views: 11030
PDF Downloads: 298
Cited By: 0

姓名
邮箱
手机号码
标题
留言内容
验证码

Search

Article

留言板