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The study of EGFR transport in single cell using an automatic method of trajectory identification

Li Hui Duan Zhao-Wen Dou Shuo-Xing Wang Peng-Ye

The study of EGFR transport in single cell using an automatic method of trajectory identification

Li Hui, Duan Zhao-Wen, Dou Shuo-Xing, Wang Peng-Ye
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  • We study the characteristics of motion modes of epidermal growth factor receptor (EGFR) trafficking in single cell, by using the automatic method of trajectory identification which is based on the directional persistence and mean square displacement analysis. Each trajectory of EGFRs is divided into four modes of motion: directional motion, super-diffusion, Brownian motion and sub-diffusion. The corresponding dynamic parameters of different modes of motion are calculated and discussed, providing an insight into intracellular trafficking of receptors.
      Corresponding author: Wang Peng-Ye, pywang@aphy.iphy.ac.cn
    • Funds: Project supported by the National Natural Science Foundation of China (Grant No. 10834014) and the National Basic Research Program of China (Grant No. 2009CB930704).
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  • [1]

    Saxton M J, Jacobson K 1997 Annu. Rev. Biophys. Biomol. Struct. 26 373

    [2]
    [3]

    Dahan M 2003 Science 302 442

    [4]

    Cui B X, Wu C B, Chen L, Ramirez A, Bearer E L, Li W P, Mobley W C, Chu S 2007 Proc. Natl. Acad. Sci. U. S. A. 104 13666

    [5]
    [6]

    Gonzalez-Gaitan M 2003 Nat. Rev. Mol. Cell Biol. 4 213

    [7]
    [8]

    Lakadamyali M, Rust M J, Zhuang X 2006 Cell 124 997

    [9]
    [10]
    [11]

    Watanabe T M, Higuchi H 2007 Biophys. J. 92 4109

    [12]
    [13]

    Brandenburg B, Zhuang X 2007 Nat. Rev. Microbiol. 5 197

    [14]
    [15]

    Sorkin A, Goh L K 2009 Exp. Cell Res. 315 683

    [16]

    Kural C, Kim H, Syed S, Goshima G, Gelfand V I, Selvin P R 2005 Science 308 1469

    [17]
    [18]
    [19]

    Ping X, Dou S X, Wang P Y 2004 Chin. Phys. 13 1569

    [20]

    Vale R D 2003 Cell 112 467

    [21]
    [22]

    Kusumi A, Nakada C, Ritchie K, Murase K, Suzuki K, Murakoshi H, Kasai R S, Kondo J, Fujiwara T 2005 Annu. Rev. Biophys. Biomol. Struct. 34 351

    [23]
    [24]

    Arcizet D, Meier B, Sackmann E, Radler J O, Heinrich D 2008 Phys. Rev. Lett. 101 248103

    [25]
    [26]
    [27]

    Owens J, Hunter A 2000 VS 00 Proceedings of the Third IEEE International Workshop on Visual Surveillance (VS2000) Dublin, Ireland, July 1, 2000 p77

    [28]

    Helmuth J A, Burckhardt C J, Koumoutsakos P, Greber U F, Sbalzarini I F 2007 J. Struc. Biol. 159 347

    [29]
    [30]
    [31]

    Verkman A S 2002 Trends Biochem. Sci. 27 27

    [32]

    Jaiswal J K, Mattoussi H, Mauro J M, Simon S M 2003 Nat. Biotechnol. 21 47

    [33]
    [34]

    Sbalzarini I F, Koumoutsakos P 2005 J. Struc. Biol. 151 182

    [35]
    [36]
    [37]

    Courty S, Luccardini C, Bellaiche Y, Cappello G, Dahan M 2006 Nano Lett. 6 1491

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  • Received Date:  18 March 2011
  • Accepted Date:  23 May 2011
  • Published Online:  20 March 2012

The study of EGFR transport in single cell using an automatic method of trajectory identification

    Corresponding author: Wang Peng-Ye, pywang@aphy.iphy.ac.cn
  • 1. Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China
Fund Project:  Project supported by the National Natural Science Foundation of China (Grant No. 10834014) and the National Basic Research Program of China (Grant No. 2009CB930704).

Abstract: We study the characteristics of motion modes of epidermal growth factor receptor (EGFR) trafficking in single cell, by using the automatic method of trajectory identification which is based on the directional persistence and mean square displacement analysis. Each trajectory of EGFRs is divided into four modes of motion: directional motion, super-diffusion, Brownian motion and sub-diffusion. The corresponding dynamic parameters of different modes of motion are calculated and discussed, providing an insight into intracellular trafficking of receptors.

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