Search

Article

x

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

A nnovel wide field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination

Wen Qiao Wang Kai-Ge Shao Yong-Hong Qu Jun-Le Niu Han-Ben

A nnovel wide field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination

Wen Qiao, Wang Kai-Ge, Shao Yong-Hong, Qu Jun-Le, Niu Han-Ben
PDF
Get Citation
Metrics
  • Abstract views:  839
  • PDF Downloads:  606
  • Cited By: 0
Publishing process
  • Received Date:  21 May 2012
  • Accepted Date:  06 September 2012
  • Published Online:  05 February 2013

A nnovel wide field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination

  • 1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
Fund Project:  Project supported by the National Natural Science Foundation of China (Grant Nos. 61108026, 61078067), Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20114408120001), Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (Grant No. LYM11107) and Science Foundation of Shenzhen University, China (Grant No. 201033).

Abstract: We propose a novel wide-field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination in this article. A new type of polarization filtering image enhancement technology, based on the differences in polarization characteristic between excitation light and fluorescence, is used to filter out the excitation light and enhance image quality. The dynamic speckle illumination is employed to achieve the wide field fluorescence sectioning which has many advantages, such as simple configuration, low cost, rapid response and easy operation. The experimental results indicate that our filtering method can be utilized to improve the image quality significantly, and the dynamic using speckle illumination can be employed to achieve optical sectioning with a wide field and a high vertical resolution capability. Our study is not only to enrich the technological method to extract the weak fluorescence signal from the strong excitation light, but also to provide a significant reference for developing a light wavelength tunable multi-spectral fluorescence microscope with rapid response.

Reference (11)

Catalog

    /

    返回文章
    返回