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Digital holographic microscope employing dual-Lloyd’s mirror

Yuan Fei Yuan Cao-Jin Nie Shou-Ping Zhu Zhu-Qing Ma Qing-Yu Li Ying Zhu Wen-Yan Feng Shao-Tong

Digital holographic microscope employing dual-Lloyd’s mirror

Yuan Fei, Yuan Cao-Jin, Nie Shou-Ping, Zhu Zhu-Qing, Ma Qing-Yu, Li Ying, Zhu Wen-Yan, Feng Shao-Tong
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Publishing process
  • Received Date:  07 November 2013
  • Accepted Date:  10 January 2014
  • Published Online:  05 May 2014

Digital holographic microscope employing dual-Lloyd’s mirror

  • 1. Key Laboratory for Opto-electronic Technology of Jiangsu Province, Nanjing Normal University, Nanjing 210023, China
Fund Project:  Project supported by the National Natural Science Foundation of China (Grant Nos. 61377003, 11274176).

Abstract: We propose a digital holographic microscopy (DHM) setup employing a configuration with two Lloyd's mirrors, which is based on self-referencing and dual-wavelength optical phase unwrapping. We use two Lloyd's mirrors to fold the beam which does not exhibit sample structure and acts as the reference beam, returning onto itself to form a dual-wavelength hologram. Two wrapped phase images for every wavelength are reconstructed by angular spectrum method. Then the wrapped phase image and the three-dimensional profile image are acquired by dual-wavelength optical unwrapping method. In the experiment, we use two lasers of different wavelengths of 532 and 632 nm to record a hologram. Numerical methods are subsequently applied to reconstruct the hologram to enable direct access to both phase and amplitude information. The quantitative experimental results with dual-wavelength DHM involve a deviation less than 5% from the calibration values. The validity of this method is demonstrated.

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