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物理学报. 2009, 58(6): 4301-4306.
doi: 10.7498/aps.58.4301.
用单分子磁镊研究顺铂导致的DNA凝聚
- 收稿日期: 2009-03-06
- 修回日期: 2009-03-24
- 刊出日期: 2009-06-20
English Abstract
Study of cisplatin-induced DNA compaction using single molecule magnetic tweezers
- Received Date:
06 March 2009
- Accepted Date:
24 March 2009
- Published Online:
20 June 2009
Abstract: We report a single molecule study on cisplatin-induced DNA compaction. It is found that, at a small external tension （<1 pN）, the DNA compaction time course is irregular with many continuous shortening processes and abrupt large size jumps. The time course becomes smooth and hyperbolic when the external tension increases to a moderate value, which is not large enough to inhibit the compaction. The time course depends on the external tension rather than the concentration of cisplatin; the latter only influences the compaction rate. The results are consistent with a looping-and-cross-linking compaction model. Namely, in aqueous solutions, cisplatin forms both monoadducts and diadducts with DNA. When cisplatin induces distant cross-links between DNA bases, micro-loops are formed, which make DNA compact. Further cross-linking between the micro-loops leads to complete compaction of DNA. In addition, the compacted DNA structure is quite stable.
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