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无透镜傅里叶变换数字全息术对活细胞进行显微相衬成像测量时,由于细胞处于运动状态导致记录物体与参考点源难以共面,从而引入成像误差.本文提出了一种有效可靠的基于位相分布的自动对焦再现算法,只需通过一幅全息图,即可确定出记录距离的优化数值解,并结合一次位相线性拟合,成功补偿掉非共面误差.将这种算法应用到活体宫颈癌细胞的相衬成像实验中获得了较为理想的实验结果,表明了这种自动补偿非共面误差算法的可行性.
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关键词:
- 数字全息 /
- 无透镜傅里叶变换全息 /
- 自动对焦 /
- 位相畸变校正
When the lensless Fourier transform digital holography is applied to the microscopic phase-contrast imaging on live cells, the motion of cells will lead to a non-coplanary phenomenon between the object recorded and the reference source. This could result in the imaging aberration. An effective and robust autofocus procedure based on the phase distribution is presented in the paper. With the initial measurement of the distance between the reference source and the hologram, the optimal parameters corresponding to the phase-contrast image can be achieved by a single hologram combined with the linearity fitting. Lensless Fourier transform digital holographic system is built and the experiments on the phase-contrast imaging of the live cervical carcinoma cells are performed. Finally, the good experimental results are obtained. Both the theoretical analysis and the experimental investigation verify the feasibility and the validity of the automatic procedure for non-coplanar aberration compensation.-
Keywords:
- digital holography /
- lensless Fourier transform holography /
- autofocus /
- phase distortion correction
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[3] Fan Z B, Li J C 2010 Chin. Phys.B 19 4
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[13] Dirksen D, Droste H, Kemper B 2001 Optics and Lasers in Engineering 36 3
[14] Pedrini G, Tiziani H J 2002 Appl. Opt. 41 22
[15] Yuan C J, Zhong L Y, Zhu Y, Wang Y P, Lü X X 2004 Laser Journal 25 5 (in Chinese) [袁操今、钟丽云、朱 越、王艳萍、吕晓旭 2004 激光杂志 25 5]
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[17] Jiang H Z, Zhao J L, Di J L, Yan X B, Sun W W 2008 Acta Optica Sin. 28 8(in Chinese) [姜宏振、赵建林、邸江磊、闫晓博、孙伟伟 2008 光学学报 28 8]
[18] Colomb T, Cuche E, Charrière F, Kühn J, Aspert N, Montfort F, Marquet P, Depeursinge C 2006 Appl. Opt. 45 5
[19] Ferraro P, Nicola S D, Finizio A, Coppola G, Grilli S, Magro C, Pierattini G 2003 Appl. Opt. 42 11
[20] Liebling M, Blu T, Cuche E, Marquer P, Depeursinge C, Unser M 2002 IEEE 0-7803-7584-X.
[21] Kemper B, Bally G 2008 Appl. Opt. 47 4
[22] Ferraro P, Alferi D 2006 Opt. Lett. 31 10
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[1] Cuche E, Marquet P, Depeursinge C 1999 App. Opt. 38 34
[2] Charrière F, Marian A, Montfort F, Kuehn J, Colomb T 2006 Opt. Lett. 31 2
[3] Fan Z B, Li J C 2010 Chin. Phys.B 19 4
[4] Liu G Y, Lü X X, Yu Q T, Zhang Q S 2009 Chin. Phys. B 18 7
[5] Yuan C J, Zhai H C, Wang X L, Wu L 2007 Acta Phys. Sin. 56 1(in Chinese)[袁操今、翟宏琛、王晓雷、吴 兰 2007 物理学报 56 1]
[6] Zhong L Y, Zhang Y M, Lü X X, Qian X F, Xiong B H 2004 Chin. J .Lasers 31 5 (in Chinese)[钟丽云、张以谟、吕晓旭、钱晓凡、熊秉衡 2004 中国激光 31 5]
[7] Shen J Y, Li X G, Chang S J, Zhang Y X 2005 Acta Phys. Sin. 54 11(in Chinese)[申金媛、李现国、常胜江、张延炘 2005物理学报 54 11]
[8] Di J L, Zhao J L, Fan Q, Jiang H Z, Sun W W 2008 Acta Optica Sin. 28 1(in Chinese)[邸江磊、赵建林、范 琦、姜宏振、孙伟伟 2008 光学学报 28 1]
[9] Wang H Y, Wang G J, Zhao J, Xie J J, Wang D Y 2007 Chin. J .Lasers 34 12(in Chinese)[王华英、王广俊、赵洁、谢建军、王大勇 2007 中国激光 34 12]
[10] Li J C, Zhang Y P, Xu W 2009 Acta Phys. Sin. 58 8 (in Chinese) [李俊昌、张亚萍、许 蔚 2009 物理学报 58 8]
[11] Wagner C, Seebacher S, Osten W 1999 Appl. Opt. 38 22
[12] Takao S, Yoneyama S, Takashi M 2002 Optics and Lasers in Engineering 38 5
[13] Dirksen D, Droste H, Kemper B 2001 Optics and Lasers in Engineering 36 3
[14] Pedrini G, Tiziani H J 2002 Appl. Opt. 41 22
[15] Yuan C J, Zhong L Y, Zhu Y, Wang Y P, Lü X X 2004 Laser Journal 25 5 (in Chinese) [袁操今、钟丽云、朱 越、王艳萍、吕晓旭 2004 激光杂志 25 5]
[16] Xie J J, Wang D Y, Wang H Y, Tao S Q 2007 Laser Journal 28 6 (in Chinese) [谢建军、王大勇、王华英、陶世荃 2007 激光杂志 28 6]
[17] Jiang H Z, Zhao J L, Di J L, Yan X B, Sun W W 2008 Acta Optica Sin. 28 8(in Chinese) [姜宏振、赵建林、邸江磊、闫晓博、孙伟伟 2008 光学学报 28 8]
[18] Colomb T, Cuche E, Charrière F, Kühn J, Aspert N, Montfort F, Marquet P, Depeursinge C 2006 Appl. Opt. 45 5
[19] Ferraro P, Nicola S D, Finizio A, Coppola G, Grilli S, Magro C, Pierattini G 2003 Appl. Opt. 42 11
[20] Liebling M, Blu T, Cuche E, Marquer P, Depeursinge C, Unser M 2002 IEEE 0-7803-7584-X.
[21] Kemper B, Bally G 2008 Appl. Opt. 47 4
[22] Ferraro P, Alferi D 2006 Opt. Lett. 31 10
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