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Inactivation of A549 cancer cells by a helium-oxygen plasma needle

Chen Wei Huang Jun Li Hui Lü Guo-Hua Wang Xing-Quan Zhang Guo-Ping Wang Peng-Ye Yang Si-Ze

Inactivation of A549 cancer cells by a helium-oxygen plasma needle

Chen Wei, Huang Jun, Li Hui, Lü Guo-Hua, Wang Xing-Quan, Zhang Guo-Ping, Wang Peng-Ye, Yang Si-Ze
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  • Received Date:  20 February 2012
  • Accepted Date:  07 March 2012
  • Published Online:  20 September 2012

Inactivation of A549 cancer cells by a helium-oxygen plasma needle

  • 1. Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Science, Beijing 100190, China;
  • 2. Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, School of Physics and Mechanical and Electrical Engineering, Xiamen University, Xiamen 361005, China
Fund Project:  Project supported by the Young Scientists Fund of the National Natural Science Foundation of China (Grant No. 11005151).

Abstract: An inactivation mechanism of A549 cancer cells is studied by using a dielectric barrier discharge (DBD) plasma needle. The influence of oxygen concentration, which is injected into helium plasma afterglow region through a stainless steel tube, is investigated. The neutral red uptake assay provides a qualitative observation of morphological differences between the dead cells and the viable cells after plasma treatment and a quantitative estimation of cell viability under different conditions. In the treatment process at a fixed power of 24 W, the inactivation efficiency of helium-oxygen plasma depends mainly on the exposure time and percentage of added oxygen in helium plasma. Experimental results show that the best parameters of the process are 150 s treatment time, 800 mL/min He with 3% O2 addition and separation of needle-to-sample 3 mm. According to the helium-oxygen emission spectra of the plasma jet, it is concluded that the reactive species (for example, OH and O) in the helium-oxygen plasma play a major role in the cell deactivation.

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