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Optical tweezers in which a tightly focused laser beam is used to trap micron-sized or nanometer-sized particles have become indispensable tools for measuring the forces and displacements associated with molecular biomechanical events in a noninvasive manner. A complete beam manipulation system is composed of a beam expander input lens, beam expander output lens, focusing lens, piezoelectric translator mirror to control the trap position, with the overfilling degree of the objective entrance aperture retained. The accurate manipulations of trap position in three dimensions are the bases for the realization of the position clamp and force clamp. The optical path of optical tweezers based on infinity corrected conjugate microscope is calculated using matrix optics. The influences on radial trap position manipulation caused by axial position adjustment of focusing lens and objective, and by the installation location error of focusing lens and piezoelectric translator mirror are analyzed. The result shows that axial position adjustment of objective introduces a nominal error in radial trap position manipulation. The misalignments of focusing lens and piezoelectric translator mirror have a greater influence on optical tweezers performances. The theory points out the accurate dynamic axial position adjustment range, which is useful to optical tweezers design and experiments.
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Keywords:
- optical tweezers /
- design /
- trap position manipulation /
- error
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[17] Yao X C, Li Z L, Guo H L, Cheng B Y, Zhang D Z 2001 Opt. Precision Eng. 1 55 (in Chinese) [姚新程, 李兆霖, 郭红莲, 程丙英, 张道中 2001 光学精密工程 1 55]
[18] Jiang Y Q, Guo H L, Liu C X, Li Z L, Cheng B Y, Zhang D Z, Jia S T 2004 Acta Phys. Sin. 53 1721 (in Chinses) [降雨强, 郭红莲, 刘春香, 李兆霖, 程丙英, 张道中, 贾锁堂 2004 物理学报 53 1721]
[19] Liu C X, Guo H L, Jiang Y Q, Li Z L, Cheng B Y, Zhang D Z 2005 Acta Phys. Sin. 54 1162 (in Chinese) [刘春香, 郭红莲, 降雨强, 李兆霖, 程丙英, 张道中 2005 物理学报 54 1162]
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[1] Clancy B E, Behnke-Parks W M, Andreasson J O L, Rosenfeld S S, Block S M 2011 Nat. Struct. Mol. Biol. 18 1020
[2] Guydosh N R, Block S M 2009 Nature 461 125
[3] Collin D, Ritort F, Jarzynski C, Smith S B, Tinoco I, Bustamante C 2005 Nature 437 231
[4] Woodside M T, Garcia-Garcia C, Block S M 2008 Curr. Opin. Chem. Biol. 12 640
[5] Qu X H, Wen J D, Lancaster L, Noller H F, Bustamante C, Tinoco I 2011 Nature 475 118
[6] Neuman K C, Nagy A 2008 Nat. Methods 5 491
[7] Phillips D B, Simpson S H, Grieve J A, Gibson G M, Bowman R, Padgett M J, Miles M J, Carberry D M 2011 Opt. Express 19 20622
[8] Preece D, Bowman R, Linnenberger A, Gibson G, Serati S, Padgett M 2009 Opt. Express 17 22718
[9] Ojala H, Korsback A, Wallin A E, Haeggstrom E 2009 Appl. Phys. Lett. 95 181104
[10] Wallin A E, Ojala H, Ziedaite G, Haeggstrom E 2011 Rev. Sci. Instrum. 82 083102
[11] Lang M J, Asbury C L, Shaevitz J W, Block S M 2002 Biophys. J. 83 491
[12] Nambiar R, Gajraj A, Meiners J C 2004 Biophys. J. 87 1972
[13] Fällman E, Axner O 1997 Appl. Opt. 36 2107
[14] Gong Z, Chen H T, Xu S H, Li Y M, Lou L R 2006 Opt. Commun. 263 229
[15] Ren Y X, Wu J G, Chen M, Li H, Li Y M 2010 Chin. Phys. Lett. 27 028703
[16] Zhou J H, Tao R Z, Hu Z B, Zhong M C, Wang Z Q, Cai J, Li Y M 2009 Chin. Phys. Lett. 26 068701
[17] Yao X C, Li Z L, Guo H L, Cheng B Y, Zhang D Z 2001 Opt. Precision Eng. 1 55 (in Chinese) [姚新程, 李兆霖, 郭红莲, 程丙英, 张道中 2001 光学精密工程 1 55]
[18] Jiang Y Q, Guo H L, Liu C X, Li Z L, Cheng B Y, Zhang D Z, Jia S T 2004 Acta Phys. Sin. 53 1721 (in Chinses) [降雨强, 郭红莲, 刘春香, 李兆霖, 程丙英, 张道中, 贾锁堂 2004 物理学报 53 1721]
[19] Liu C X, Guo H L, Jiang Y Q, Li Z L, Cheng B Y, Zhang D Z 2005 Acta Phys. Sin. 54 1162 (in Chinese) [刘春香, 郭红莲, 降雨强, 李兆霖, 程丙英, 张道中 2005 物理学报 54 1162]
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