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Abstract: The photoluminescence and energy transfer properties based on an organic salt trans-4-[p-(N-ethyl-N-(hydroxylethyl)amino)phenylstyryl]-N-(hydroxyethyl)pyridinium iodide (ASPI) doped PVK thin film were investigated by steady-state and time-resolved fluorescent spectra. We observed that the emission intensity and the fluorescent lifetime of PVK reduced with increasing ASPI doping concentration due to the Forster energy transfer from PVK to ASPI, which is strongly correlated with an efficient spectral overlap between ASPI absorption and PVK emission. We also added some Alq3 into the doping system as energy carriers in order to increase the energy transfer efficiency from PVK to ASPI at low concentration, or there will be an evident decrease in photoemission efficiency due to the concentration quenching at high concentration. The dynamical process of energy transfer is discussed.