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微流芯片中消逝波激励的荧光辐射特性研究

储玉飞 张远宪 刘春 普小云

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微流芯片中消逝波激励的荧光辐射特性研究

储玉飞, 张远宪, 刘春, 普小云

Fluorescence radiation characteristics based on evanescent wave pumping in a microfluidic chip

Chu Yu-Fei, Zhang Yuan-Xian, Liu Chun, Pu Xiao-Yun
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  • 将石英裸光纤植入聚二甲基硅氧烷基片的微流道中,采用沿光纤轴向光抽运、消逝场激励染料分子的方式,在基片微流道中获得均匀的荧光辐射.实验发现,荧光辐射的强度随光纤轴向距离的增加而衰减,光纤包层溶液折射率越大,荧光沿光纤轴向的衰减越突出;包层溶液中染料浓度越大,荧光沿光纤轴向的衰减也越突出;通过选择适当的包层溶液折射率以及染料浓度可以获得沿光纤轴向接近均匀的荧光辐射.用消逝波激励荧光的辐射理论计算了荧光光强沿光纤轴向的变化,计算结果与实验符合较好.在此基础上,设计并制作了一种具有三个通道的聚二甲基硅氧烷基片,通过在三个微流道中分别注入染料浓度均为0.1 mmol的罗丹明640、 罗丹明B 及罗丹明6 G的乙醇染料溶液,采用沿光纤轴向消逝波光激励方式,在一块聚二甲基硅氧烷基片上同时实现了三个不同波段的荧光辐射.
    A bare quartz optical fiber is implanted in a microfluidic channel of polydimethylsiloxane (PDMS) substrate. Pumping the microfluid by a continuous wave laser with a wavelength of 532 nm along the fiber axis, the fluorescent spectra from the channel filled with lower refractive index (RI) dye solution are obtained. Due to the fact that the evanescent field of the pump beam is homogeneous around fiber, the fluorescent emission from the rim of fiber is uniform. It is found experimentally that the fluorescent emission intensity decreases with the axial distance of fiber, and the intensity is very sensitive to the RI of the dye solution and the dye concentration. For the dye solution with a large RI, the emitted fluorescent intensity attenuates along the fiber axis more obviously than that of the dye solution with a small RI. For the high dye concentration solution, the emitted fluorescent intensity attenuates along the fiber axis also more significantly than that of the low dye concentration solution. Therefore, it is possible to obtain a uniform fluorescence radiation along the fiber axis by selecting a suitably smaller RI and a lower dye concentration solution. The observed experimental phenomena are well explained based on the mechanism of evanescent wave pumping fluorescent radiation. Based on the features of fluorescent emission in the microfluidic chip, a PDMS chip with three micro-channels is designed and fabricated. After injecting ethanol solutions of rhodamine 640, rhodamine B and rhodamine 6 G separately into the three channels and pumpingthese solutions by evanescent wave along the optical fiber axis, three fluorescence emissions with different wavelength ranges are successfully observed in a single PDMS chip.
      通信作者: 普小云, xypu@163.com
    • 基金项目: 国家自然科学基金(批准号:11404282,61465014)、中国科学院西部之光人才培养项目和云南大学中青年骨干教师培养项目资助的课题.
      Corresponding author: Pu Xiao-Yun, xypu@163.com
    • Funds: Project supported by the National Natural Science Foundation of China (Grant Nos. 11404282, 61465014), the Chinese Academy of Sciences “Light of West China” Program, and the Young Backbone Teachers Training Program of Yunnan University, China.
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    Vezenov D V, Mayers B T, Wolfe D B, Whitesides G M 2005 Appl. Phys. Lett. 86 041104

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    Mayers B T, Vezenov D V, Vullev V I, Whitesides G M 2005 Anal. Chem. 77 1310

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    Sun Y, Shopova S I, Wu C S, Amold S, Fan X D 2010 Proc. Natl. Acad. Sci. USA 107 16039

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    Pilgyu K, Perry S, Xavier S, Dakota O D, David E 2015 Sci. Reports 5 12087

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  • [1]

    Thorsen T, Maerkl S J, Quake S R 2002 Science 298 580

    [2]

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    [3]

    Manz A, Graber N, Widmer H 1990 Sensors and Actuators B: Chemical 1 244

    [4]

    Psaltis D, Quake S R, Yang C 2006 Nature 442 27

    [5]

    Helbo B, Kristensen A, Menon A 2003 J. Micromech. Microengin. 13 2

    [6]

    Monat C, Domachuk P, Eggleton P B 2007 Nat. Photon. 1 106

    [7]

    Chen Y C, Chen Q S, Fan X 2016 Lab on Chip 16 2228

    [8]

    Gilardi G, Beccherelli R 2013 J. Phys. D: Appl. Phys. 46 105104

    [9]

    Li M, Zhi M, Zhu H, Wu W Y, Xu Q H, Jhon M H, Chan Y 2015 Nat. Commun. 6 1

    [10]

    Fan X, Yun S H 2014 Nat. Methods 11 141

    [11]

    Zhang J, Wang S, Liu K, Wei Y, Wang X, Duan Y 2015 Anal. Chem. 87 2959

    [12]

    Lim J M, Kim S H, Yang S M 2011 Microfluid. Nanofluid. 10 211

    [13]

    Wolfe D B, Conroy R S, Garstecki P, Mayers B T, Fischbach M A, Paul M P, Whitesides G M 2004 Proc. Natl. Acad. Sci. USA 101 12434

    [14]

    Vezenov D V, Mayers B T, Wolfe D B, Whitesides G M 2005 Appl. Phys. Lett. 86 041104

    [15]

    Lim J M, Kim S H, Choi J H, Yang S M 2008 Lab on Chip 8 1580

    [16]

    Moon H J, Chough Y T, An K 2000 Phys. Rev. Lett. 85 15

    [17]

    Zhang Y X, Pu X Y, Zhu K, Feng L 2011 J. Opt. Soc. Am. B 28 2048

    [18]

    Zhu K, Zhou L, You H H, Jiang N, Pu X Y 2011 Acta Phys. Sin. 60 054205 (in Chinese) [祝昆, 周丽, 尤洪海, 江楠, 普小云 2011 物理学报 60 054205]

    [19]

    Ulrich B 1986 Lambda Chrome Laser Dyes (Lambda: Lambda Physik Gmbh)

    [20]

    Mayers B T, Vezenov D V, Vullev V I, Whitesides G M 2005 Anal. Chem. 77 1310

    [21]

    Sun Y, Shopova S I, Wu C S, Amold S, Fan X D 2010 Proc. Natl. Acad. Sci. USA 107 16039

    [22]

    Pilgyu K, Perry S, Xavier S, Dakota O D, David E 2015 Sci. Reports 5 12087

    [23]

    Fan X D, White I M 2011 Nat. Photon. 5 591

    [24]

    Mellors J S, Jorabchi K, Smith L M, Ramsey M 2010 Anal. Chem. 82 967

    [25]

    Wu D, Luo Y, Zhou X M, Dai Z P 2005 Electrophoresis 26 1

    [26]

    Vasdekis A E, Laporte G P J 2013 Int. J. Mol. Sci. 12 8

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出版历程
  • 收稿日期:  2016-11-21
  • 修回日期:  2017-03-02
  • 刊出日期:  2017-05-05

微流芯片中消逝波激励的荧光辐射特性研究

  • 1. 云南大学物理系, 昆明 650091
  • 通信作者: 普小云, xypu@163.com
    基金项目: 国家自然科学基金(批准号:11404282,61465014)、中国科学院西部之光人才培养项目和云南大学中青年骨干教师培养项目资助的课题.

摘要: 将石英裸光纤植入聚二甲基硅氧烷基片的微流道中,采用沿光纤轴向光抽运、消逝场激励染料分子的方式,在基片微流道中获得均匀的荧光辐射.实验发现,荧光辐射的强度随光纤轴向距离的增加而衰减,光纤包层溶液折射率越大,荧光沿光纤轴向的衰减越突出;包层溶液中染料浓度越大,荧光沿光纤轴向的衰减也越突出;通过选择适当的包层溶液折射率以及染料浓度可以获得沿光纤轴向接近均匀的荧光辐射.用消逝波激励荧光的辐射理论计算了荧光光强沿光纤轴向的变化,计算结果与实验符合较好.在此基础上,设计并制作了一种具有三个通道的聚二甲基硅氧烷基片,通过在三个微流道中分别注入染料浓度均为0.1 mmol的罗丹明640、 罗丹明B 及罗丹明6 G的乙醇染料溶液,采用沿光纤轴向消逝波光激励方式,在一块聚二甲基硅氧烷基片上同时实现了三个不同波段的荧光辐射.

English Abstract

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