H-22细胞声孔效应的应力阈值

莫润阳; 林书玉; 王成会

doi:10.7498/aps.60.114306

摘要

理论和实验研究了超声空化场中的H-22型肝癌细胞产生可逆声孔效应的剪应力阈值.本文用1.37 MHz的聚焦声场,当超顺磁性纳米氧化铁在细胞悬液中的终浓度为410 g/mL,换能器负载电功率为2 W,超声辐照60 s,细胞存活率90%以上时,有45.913.5%的细胞显示普鲁士蓝染阳性,暗示超声作用下,这些细胞表面曾出现可逆性微孔而使磁性微粒由此进入细胞内.利用无界自由空间微泡运动方程的球对称稳态解对实验条件下细胞膜表面的切变应力进行数值估算,结果表明,使H-22细胞产生可逆性声孔效应的微流剪应力阈值为697 Pa.

关键词:

声孔效应 /
磁性标记 /
微流 /
剪应力

Abstract

Based on a spherically symmetric bubble model, the threshold shear stress for H-22 cells which are irradiated by continuous focused ultrasound is estimated. On the condition that the final concentration of the superparamagnetic iron oxide (SPIO) in the cell suspension is 410 g/mL, the focused ultrasound sonication is at a frequency of 1.37 MHz, the power from amplifer is 2 W, and H-22 cells are exposed to the ultrasound for 60 s, the labeling efficiency is about 45.9%13.5%. Prussian blue staining confirms iron uptake and shows numerous blue-stained iron particles in the cytoplasm, while more than 90% labeled cells remained viable. The results show that the reparable sonoporation arises in plasma membrane and SPIO nanoparticles enter into the H-22 cells. Numerical calculations show that the shear stress acting on the cell is 697 Pa, which is the threshold shear stress for H-22 cell sonoporation.

Keywords:

作者及机构信息

1.
陕西师范大学物理学与信息技术学院,陕西省超声学重点实验室,西安 710062

基金项目: 国家自然科学基金(批准号: 10974128)、陕西省自然科学基金(批准号:2010JM1002)和中央高校基本科研业务费专项资金 (批准号:GK201002009)资助的课题.

Authors and contacts

1.
Shaanxi Key Laboratory of Ultrasonic, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China

参考文献

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施引文献

[1]	Miller D L, Bao S, Morris J E 1999 Ultrasound Med. Biol. 25 143
[2]
[3]	Zhang C B, Qiu Y Y, Xi X Y, Zhang D 2009 Acta Phys. Sin. 58 3396(in Chinese) [张春兵、邱媛媛、郗晓宇、章东 2009 物理学报 58 3396]
[4]
[5]	Huber P E, Pfisterer P 2000 Gene. Ther. 7 1516
[6]
[7]	Wu J 2002 Ultrasound Med. Biol.28 125
[8]
[9]	Whelan J 2002 Drug Delivery Today 7 585
[10]	Mo R Y, Wang G Z 2008 Acta Biophys. Sin. 24 445(in Chinese) [莫润阳、王公正 2008 生物物理学报 24 445]
[11]
[12]	Cheng J C, Liang B, Zhu Z M 2006 Chin. Phys. 15 412
[13]
[14]	Wu J, Nyborg W L 2008 Adv. Drug Deliver. Rev. 60 1103
[15]
[16]
[17]	Wu J, Ross J R, Chiu J F 2002 J. Acoust. Soc. Am. 111 1460
[18]
[19]	Wamel A van, Kooiman K, Harteveld M 2006 J. Control Release 112 149
[20]	Prenticel P, Cuschieri A, Dholakia K, Prausnitz M, Campbell P 2005 Nature Physics 1 107
[21]
[22]
[23]	Ohl C D, Arora M, Lkink R, Jong N, Versluis M, Delius M, Lohse D 2006 Biophys. J. 91 4285
[24]	Lewin P A, Leif Bjrn 1982 J. Acoust. Soc. Am.71 728
[25]
[26]	Wu E L, Wang P, Wang X B, Qi H, Liu Q H 2006 Appl. Acoust. 25 121(in Chinese) [吴二林、王攀、王筱冰、齐浩、刘全宏 2006 应用声学 25
[27]
[28]	121 McNeil P L 1989 Methods Cell Biol. 29 153
[29]
[30]
[31]	Gracewski S M, Miao H Y, Dalecki D 2005 J. Acoust. Soc. Am. 117 1140
[32]	Chen Q, Zou X H, Cheng J C 2006 Acta Phys. Sin. 55 6476(in Chinese) [陈谦、邹欣晔、程建春 2006 物理学报 55 6476]
[33]
[34]
[35]	Wu J 2007 Prog. in Biophys. and Mole. Biol. 93 363
[36]
[37]	Long M,Wu Z Z,Wang H B, Song G B,Wang X H,Wu Y P 1996 Acta Biophys. Sin.12 169(in Chinese) [龙勉、吴泽志、王红兵、宋关斌、王宪航、吴云鹏 1996生物物理学报 12 169]
[38]	Rooney A 1972 J. Acoust. Soc. Am.52 1718
[39]

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