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We successfully label-free and real-time detecte the interactional dynamic processes of rabbit IgG with different concentrations (1.25 mg/mL, 2.50 mg/mL and 5.00 mg/mL), and goat anti-rabbit IgG with a concentration of 0.02 mg/mL by the oblique-incidence reflectivity difference method (OIRD). The dynamic curves and times of interaction, response to concentration of rabbit IgG, are obtained. The experimental results demonstrate that the OIRD method can not only can distinguish the biomolecular interactions, but also real-time detect the interactional dynamic processes of biomolecules, indicating that the OIRD method has promising and extensive applications for the investigating of biomolecular interactions.
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Keywords:
- oblique-incidence reflectivity difference method /
- label-free and real-time detection /
- interaction dynamic process of proteins
[1] Zhu H, Bilgin M, Bangham R, Hall D, Casamayor A, Bertone P, Lan N, Jansen R, Bidlingmaier S, Houfek T, itchell T, Miller P, Dean R A, Gerstein M, Snyder M 2001 Science 293 2101
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[3] Yu X B, Xu D K, Cheng Q 2006 Proteomics 6 5493
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[6] Gao X, Cui Y, Levenson R M, Chung L W K, Nie S M 2004 Nat. Biotechnol. 22 969
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[9] Fei Y Y, Landry J P, Sun Y S, Zhu X D, Luo J T,Wang X B, Lam K S 2008 Rev. Sci. Instrum. 79 013708
[10] Wang X, Yuan K, Lu H, Wen J, Lu H B, Jin K J, Zhou Y L, Yang G Z, Li W, Ruan K C 2010 J. Appl. Phys. 107 063109
[11] Wang X, Lu H, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2010 Chin. Phys. Lett. 27 107801
[12] Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Ruan K C, Yang G Z 2010 J. Opt. 12 095301
[13] Yuan K, Wang X, Lu H, Wen J, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1434
[14] Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1230
[15] Wen J, Lu H, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 306
[16] Wang X, Lu H, Dai J, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2011 Chin. Phys. B 20 010704
[17] Zhang H Y, Lu H, Li W, Liang R Q, Jin K J, Zhou Y L, Ruan K C, Yang G Z 2008 Chin. Phys. B 17 2288
[18] Zhu X D, Fei Y Y, Wang X, Lu H B, Yang G Z 2007 Phys. Rev. B 75 245434
[19] Liang R Q, Tan C Y, Ruan K C 2004 J. Imminol. Methods 285 157
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[1] Zhu H, Bilgin M, Bangham R, Hall D, Casamayor A, Bertone P, Lan N, Jansen R, Bidlingmaier S, Houfek T, itchell T, Miller P, Dean R A, Gerstein M, Snyder M 2001 Science 293 2101
[2] Espina V,Woodhouse E C, Wulfkuhle J, Asmussen H D, Petricoin E F, Liotta L A 2004 J. Immunol. Methods 290 121
[3] Yu X B, Xu D K, Cheng Q 2006 Proteomics 6 5493
[4] Ray S, Mehta G, Srivastava S 2010 Proteomics 10 731
[5] Han M, Gao X, Su J Z, Nie S 2001 Nat. Biotechnol. 19 631
[6] Gao X, Cui Y, Levenson R M, Chung L W K, Nie S M 2004 Nat. Biotechnol. 22 969
[7] Li P Y, Lin B, Gerstenmaier J, Cunningham B T 2004 Sens. Actuat. B 99 6
[8] Zhu X D, Landry J P, Sun Y S, Gregg J P, Lam K S, Guo X W 2007 Appl. Opt. 46 1890
[9] Fei Y Y, Landry J P, Sun Y S, Zhu X D, Luo J T,Wang X B, Lam K S 2008 Rev. Sci. Instrum. 79 013708
[10] Wang X, Yuan K, Lu H, Wen J, Lu H B, Jin K J, Zhou Y L, Yang G Z, Li W, Ruan K C 2010 J. Appl. Phys. 107 063109
[11] Wang X, Lu H, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2010 Chin. Phys. Lett. 27 107801
[12] Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Ruan K C, Yang G Z 2010 J. Opt. 12 095301
[13] Yuan K, Wang X, Lu H, Wen J, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1434
[14] Lu H, Wen J, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 1230
[15] Wen J, Lu H, Wang X, Yuan K, Lu H B, Zhou Y L, Jin K J, Yang G Z, LiW, Ruan K C 2010 Sci. China Mech. Astron. 53 306
[16] Wang X, Lu H, Dai J, Wen J, Yuan K, Lu H B, Jin K J, Zhou Y L, Yang G Z 2011 Chin. Phys. B 20 010704
[17] Zhang H Y, Lu H, Li W, Liang R Q, Jin K J, Zhou Y L, Ruan K C, Yang G Z 2008 Chin. Phys. B 17 2288
[18] Zhu X D, Fei Y Y, Wang X, Lu H B, Yang G Z 2007 Phys. Rev. B 75 245434
[19] Liang R Q, Tan C Y, Ruan K C 2004 J. Imminol. Methods 285 157
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