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研究了大气压冷等离子体射流对子宫颈癌Hela细胞的灭活机制. 在倒置显微镜下观察不同等离子体处理条件下的细胞形态, 并通过中性红吸收测试定量测定各个条件下的细胞存活率. 将功率维持在18 W, 在900 mL/min 氩等离子体中添入氧气的百分含量分别为1%, 2%, 4% 和8%的条件下处理Hela细胞, 探讨活性气体氧气在惰性气体氩气中的百分含量对Hela癌细胞灭活效率的影响, 发现添加2%氧气时, 氩/氧等离子体灭活效果最佳, 处理180 s后细胞存活率可降至7%. 当继续添加氧超过2%时, 灭活效果逐渐减弱, 直至8%时, 其效果反而不如单纯氩等离子体. 通过测量等离子体发射光谱, 结果表明活性氧自由基在癌细胞灭活过程中可能起关键作用.
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关键词:
- 大气压冷等离子体射流 /
- Hela癌细胞 /
- 存活率 /
- 发射光谱
An inactivation mechanism study on Hela cancer cells by means of an atmospheric pressure cold plasma jet is presented. Cell morphology is observed under an inverted microscope after plasma treatment. The neutral red uptake assay provides quantitative evaluations of cell viability under different conditions. The effect of the inactivation efficiency of Hela cancer cells in the argon (900 mL/min) with addition of different amount of oxygen (1%, 2%, 4%, 8%) into atmospheric pressure cold plasma jet is discussed under the fixed power 18 W. Results show that 2% O2 addition provides the best inactivation efficiency, and the survival rate can be reduced to 7% after 180 s treatment. When the oxygen addition exceeds 2%, the inactivation efficiency gradually weakens. The effect is not so good as that in pure argon plasma when the oxygen addition arrives at 8%. According to the emission spectrum of the plasmum, it is concluded that the reactive oxygen species in the plasma play a key role in cancer cell inactivation process.-
Keywords:
- atmospheric pressure cold plasma jet /
- Hela cancer cells /
- survival rate /
- optical emission spectra
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[1] Sladek R E J, Stoffels E, Walraven R, Tielbeek P J A, Koolhoven R A 2004 IEEE Trans. Plasma Sci. 32 1540
[2] Zhang X H, Li M J, Zhou R L, Feng K C, Yang S Z 2008 Appl. Phys. Lett. 93 021502
[3] Lee H W, Kim G J, Kim J M, Park J K, Lee J K, Kim G C 2009 Endod. J. 35 587
[4] Chen W, Huang J, Li H, L G H, Wang X Q, Zhang G P, Wang P Y, Yang S Z 2012 Acta Phys. Sin. 61 185203 (in Chinese) [陈维, 黄骏, 李辉, 吕国华, 王兴权, 张国平, 王鹏业, 杨思泽 2012 物理学报 61 185203]
[5] Zhang X H, Huang J, Liu X D, Peng L, Sun Y, Chen W, Feng K C, Yang S Z 2009 Acta Phys. Sin. 58 1595 (in Chinese) [张先徽, 黄骏, 刘筱娣, 彭磊, 孙岳, 陈维, 冯克成, 杨思泽 2009 物理学报 58 1595]
[6] Xiong Z L, Cao Y G, Lu X P, Du T 2011 IEEE Trans. Plasma Sci. 39 2968
[7] Keidar M, Walk R, Shashurin A, Srinivasan P, Sandler A, Dasgupta S, Ravi R, Guerrero-Preston R, Trink B 2011 British Journal of Cancer 105 1295
[8] Huang J, Chen W, Li H, Wang X Q, L G H, Latif Khosa M, Guo M, Feng K C, Wang P Y, Yang S Z 2011 J. Appl. Phys. 109 053305
[9] Huang J, Li H, Chen W, L G H, Wang X Q, Zhang G P, Ostrikov K, Wang P Y, Yang S Z 2011 Appl. Phys. Lett. 99 253701
[10] Iseki S, Nakamura K, Hayashi M, Tanaka H, Kondo H, Kajiyama H, Kano H, Kikkawa F, Hori M 2012 Appl. Phys. Lett. 100 113702
[11] Chen W, Huang J, Du N, Liu X D, Wang X Q, L G H, Zhang G P, Guo L H, Yang S Z 2012 J. Appl. Phys. 112 013304
[12] Chen W, Huang J, Du N, Liu X D, L G H, Wang X Q, Zhang G P, Guo L H, Yang S Z 2012 Chin. Phys. Lett. 29 075203
[13] Zhang X H, Huang J, Liu X D, Peng L, Guo L H, L G H, Chen W, Feng K C, Yang S Z 2009 J. Appl. Phys. 105 063302
[14] Chen G L, Zheng X, L G H, Zhang Z X, Sylvain M, Wilson S, Michael T, Yang S Z 2012 Chin. Phys. B 21 105201
[15] Deng S X, Cheng C, Ni G H, Meng Y D, Chen H 2010 Chin. Phys. B 19 105203
[16] Valdespino V M, Valdespino V E 2006 Curr. Opin Obstet Gynecol. 18 35
[17] Yang L, Parkin D M, Ferlay J, Ferlay J, Li L, Chen Y D 2005 Cancer Epidemiology, Biomarkers and Prevention 14 243
[18] Massad L S, Xie X, Darragh T M, Minkoff H, Levine A M, D'Souza G, Cajigas A, Colie C, Watts D H, Strickler H 2009 Obstet Gynecol. 114 1063
[19] Bondar L, Hoogeman M S, Vásquez Osorio E M, Heijmen B J 2010 Med. Phys. 37 3760
[20] Parkin D M, Bray F I, Devesa S S 2001 Eur. J. Cancer 37 S4
[21] Domingues A P, Mota F, Durao M, Frutuoso C, Amaral N, de Oliveira C F 2010 International Journal of Gynecological Cancer 20 294
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