Inactivation of A549 cancer cells by a helium-oxygen plasma needle

Chen Wei; Huang Jun; Li Hui; Lü Guo-Hua; Wang Xing-Quan; Zhang Guo-Ping; Wang Peng-Ye; Yang Si-Ze

doi:10.7498/aps.61.185203

Abstract

An inactivation mechanism of A549 cancer cells is studied by using a dielectric barrier discharge (DBD) plasma needle. The influence of oxygen concentration, which is injected into helium plasma afterglow region through a stainless steel tube, is investigated. The neutral red uptake assay provides a qualitative observation of morphological differences between the dead cells and the viable cells after plasma treatment and a quantitative estimation of cell viability under different conditions. In the treatment process at a fixed power of 24 W, the inactivation efficiency of helium-oxygen plasma depends mainly on the exposure time and percentage of added oxygen in helium plasma. Experimental results show that the best parameters of the process are 150 s treatment time, 800 mL/min He with 3% O2 addition and separation of needle-to-sample 3 mm. According to the helium-oxygen emission spectra of the plasma jet, it is concluded that the reactive species (for example, OH and O) in the helium-oxygen plasma play a major role in the cell deactivation.

Keywords:

Authors and contacts

1.
Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Science, Beijing 100190, China;
2.
Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, School of Physics and Mechanical and Electrical Engineering, Xiamen University, Xiamen 361005, China
Funds: Project supported by the Young Scientists Fund of the National Natural Science Foundation of China (Grant No. 11005151).

References

[1]	Laroussi M 2005 Plasma Process. Polym. 2 391
[2]	Fridman G, Brooks A, Galasubramanian M 2007 Plasma Process. Polym. 4 370
[3]	Deng X T, Shi J J, Kong M G 2006 IEEE Trans. Plasma Sci. 34 1310
[4]	Fridman G, Friedman G, Gutsol A 2008 Plasma Process. Polym. 5 503
[5]	Lee H W, Kim G J, Kim J M 2009 Endod J. 35 587
[6]	Fridman G, Shereshevsky A, Jost M M 2007 Plasma Chem. Plasma Process. 27 163
[7]	Zhang X H, Li M J, Zhou R L 2008 Appl. Phys. Lett. 93 021502
[8]	Zhang X H, Huang J, Liu X D 2009 Acta Phys. Sin. 58 1595 (in Chinese) [张先徽, 黄骏, 刘筱娣 2009 物理学报 58 1595]
[9]	Guillermo R, Ana D P, Jorge L Z 2008 Nature Protocols 3 1125
[10]	Jacob A E, Hobbs S J 1974 J. Bacteriol. 117 360
[11]	Andrade F J, Wetzel W C, Chan G C 2006 J. Anal. At. Spectrom. 21 1175
[12]	Alvarez R, Quintero M C, Rodero A 2005 J. Phys. D 38 3768
[13]	Naidis G V 1997 J. Phys. D 30 1214
[14]	Leveille V, Coulombe S 2005 Plasma Sources Sci. Technol. 14 467
[15]	Lee Y H, Yi C H, Chung M J 2001 Surf. Coat. Technol. 146-147 474
[16]	Seo D C, Chung T H 2001 J. Phys. D 34 2854
[17]	Dreher D, Junod A F 1996 Eur. J. Cancer 32A 30
[18]	Halliwell B, Aruoma O I 1991 FEBS Lett. 281 9

Cited By

[1]	Laroussi M 2005 Plasma Process. Polym. 2 391
[2]	Fridman G, Brooks A, Galasubramanian M 2007 Plasma Process. Polym. 4 370
[3]	Deng X T, Shi J J, Kong M G 2006 IEEE Trans. Plasma Sci. 34 1310
[4]	Fridman G, Friedman G, Gutsol A 2008 Plasma Process. Polym. 5 503
[5]	Lee H W, Kim G J, Kim J M 2009 Endod J. 35 587
[6]	Fridman G, Shereshevsky A, Jost M M 2007 Plasma Chem. Plasma Process. 27 163
[7]	Zhang X H, Li M J, Zhou R L 2008 Appl. Phys. Lett. 93 021502
[8]	Zhang X H, Huang J, Liu X D 2009 Acta Phys. Sin. 58 1595 (in Chinese) [张先徽, 黄骏, 刘筱娣 2009 物理学报 58 1595]
[9]	Guillermo R, Ana D P, Jorge L Z 2008 Nature Protocols 3 1125
[10]	Jacob A E, Hobbs S J 1974 J. Bacteriol. 117 360
[11]	Andrade F J, Wetzel W C, Chan G C 2006 J. Anal. At. Spectrom. 21 1175
[12]	Alvarez R, Quintero M C, Rodero A 2005 J. Phys. D 38 3768
[13]	Naidis G V 1997 J. Phys. D 30 1214
[14]	Leveille V, Coulombe S 2005 Plasma Sources Sci. Technol. 14 467
[15]	Lee Y H, Yi C H, Chung M J 2001 Surf. Coat. Technol. 146-147 474
[16]	Seo D C, Chung T H 2001 J. Phys. D 34 2854
[17]	Dreher D, Junod A F 1996 Eur. J. Cancer 32A 30
[18]	Halliwell B, Aruoma O I 1991 FEBS Lett. 281 9

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Vol. 61, Issue 18 - 2012-09-20

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