-
In a fluorescent nano-resolution microscope based on single molecular localization, drift of focal plane will bring an additional deviation to the accuracy of single molecular localization. Consequently, this will reduce the final resolution of the reconstructed image and cause image degradation. Therefore, it is vital to control the system drift to a minimum level as much as possible. In recent years, the anti-drift ways emerged in endlessly. In this paper we made a systematic study aiming at the method in which optical measurement and negative feedback control are used. The basic principle and its implementation of the system are analyzed, and possible error is also evaluated. Finally, the precision of the system is tested experimentally. With this device, axial drift can be detected and corrected automatically in time, and the axial anti-drift accuracy as high as 9.93 nm can be achieved, which is one order higher than that of the existing commercial microscopies.
-
Keywords:
- nano-resolution /
- quadrant detector /
- axial anti-drift
[1] Huang B, Wang W, Bates M, Zhuang X 2008 Science 319 810
[2] Pavani S R P, Thompson M A, Biteen J S, Lord S J, Liu N, Twieg R J, Piestun R, Moerner W 2009 PNAS 106 2995
[3] Juette M F, Gould T J, Lessard M D, Mlodzianoski M J, Nagpure B S, Bennett B T, Hess S T, Bewersdorf J 2008 Nat. Methods 5 527
[4] Rust M J, Bates M, Zhuang X 2006 Nat. Methods 3 793
[5] Chen D N, Liu L, Yu B, Niu H B 2010 Acta Phys. Sin. 59 6948 (in Chinese) [陈丹妮, 刘磊, 于斌, 牛憨笨 2010 物理学报 59 6948]
[6] Shtengel G, Galbraith J A, Galbraith C G, Lippincott-Schwartz J, Gillette J M, Manley S, Sougrat R, Waterman C M, Kanchanawong P, Davidson M W 2009 PNAS 106 3125
[7] Ram S, Prabhat P, Ward E S, Ober R J 2009 Opt. Express 17 6881
[8] Li H, Yu B, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 124201 (in Chinese) [李恒, 于斌, 陈丹妮, 牛憨笨 2013 物理学报 62 124201]
[9] Yu B, Li H, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 154206 (in Chinese) [于斌, 李恒, 陈丹妮, 牛憨笨 2013 物理学报 62 154206]
[10] Shroff H, Galbraith C G, Galbraith J A, Betzig E 2008 Nat. Methods 5 417
[11] Bates M, Huang B, Dempsey G T, Zhuang X 2007 Science 317 1749
[12] Gould T J, Verkhusha V V, Hess S T 2009 Nat. Protoc. 4 291
[13] Peters J 2008 Nat. Methods, Application Notes
[14] Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S, Davidson M W, Lippincott-Schwartz J, Hess H F 2006 Science 313 1642
[15] Carter A R, King G M, Ulrich T A, Halsey W, Alchenberger D, Perkins T T 2007 Appl. Opt. 46 421
[16] Lee S H, Baday M, Tjioe M, Simonson P D, Zhang R, Cai E, Selvin P R 2012 Opt. Express 20 12177
[17] Mcgorthy R, Kamiyama D, Huang B 2013 Opt. Nanosc. 2 3
[18] Wade A, Fitzke F 1998 Opt. Express 3 190
[19] Mlodzianoski M J, Schreiner J M, Callahan S P, Smolková K, Dlasková A, Šantorová J, Ježek P, Bewersdorf J 2011 Opt. Express 19 15009
-
[1] Huang B, Wang W, Bates M, Zhuang X 2008 Science 319 810
[2] Pavani S R P, Thompson M A, Biteen J S, Lord S J, Liu N, Twieg R J, Piestun R, Moerner W 2009 PNAS 106 2995
[3] Juette M F, Gould T J, Lessard M D, Mlodzianoski M J, Nagpure B S, Bennett B T, Hess S T, Bewersdorf J 2008 Nat. Methods 5 527
[4] Rust M J, Bates M, Zhuang X 2006 Nat. Methods 3 793
[5] Chen D N, Liu L, Yu B, Niu H B 2010 Acta Phys. Sin. 59 6948 (in Chinese) [陈丹妮, 刘磊, 于斌, 牛憨笨 2010 物理学报 59 6948]
[6] Shtengel G, Galbraith J A, Galbraith C G, Lippincott-Schwartz J, Gillette J M, Manley S, Sougrat R, Waterman C M, Kanchanawong P, Davidson M W 2009 PNAS 106 3125
[7] Ram S, Prabhat P, Ward E S, Ober R J 2009 Opt. Express 17 6881
[8] Li H, Yu B, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 124201 (in Chinese) [李恒, 于斌, 陈丹妮, 牛憨笨 2013 物理学报 62 124201]
[9] Yu B, Li H, Chen D N, Niu H B 2013 Acta Phys. Sin. 62 154206 (in Chinese) [于斌, 李恒, 陈丹妮, 牛憨笨 2013 物理学报 62 154206]
[10] Shroff H, Galbraith C G, Galbraith J A, Betzig E 2008 Nat. Methods 5 417
[11] Bates M, Huang B, Dempsey G T, Zhuang X 2007 Science 317 1749
[12] Gould T J, Verkhusha V V, Hess S T 2009 Nat. Protoc. 4 291
[13] Peters J 2008 Nat. Methods, Application Notes
[14] Betzig E, Patterson G H, Sougrat R, Lindwasser O W, Olenych S, Bonifacino J S, Davidson M W, Lippincott-Schwartz J, Hess H F 2006 Science 313 1642
[15] Carter A R, King G M, Ulrich T A, Halsey W, Alchenberger D, Perkins T T 2007 Appl. Opt. 46 421
[16] Lee S H, Baday M, Tjioe M, Simonson P D, Zhang R, Cai E, Selvin P R 2012 Opt. Express 20 12177
[17] Mcgorthy R, Kamiyama D, Huang B 2013 Opt. Nanosc. 2 3
[18] Wade A, Fitzke F 1998 Opt. Express 3 190
[19] Mlodzianoski M J, Schreiner J M, Callahan S P, Smolková K, Dlasková A, Šantorová J, Ježek P, Bewersdorf J 2011 Opt. Express 19 15009
Catalog
Metrics
- Abstract views: 6947
- PDF Downloads: 293
- Cited By: 0
下载:
